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historically hasn't counting elements in the genome been an unreasonably effective way in figuring out the info in the genome? I could simply count occurrences and location of the following 180bp sequence and experiments based on that strategy would teach me tons:pic.twitter.com/GtthGhLY9a
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Really nice use of OLS to arrive at the following best fitted model of the vS.pic.twitter.com/LkiBNUWRYO
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Im starting to have problems keeping up with the literature so a little bit late on this preprint but looks really great. Projection-specific rabies tracing of ventral subiculum by
@RyanWee in@MacAskillAF lab. Love the use of stat modelling in this paper! https://twitter.com/RyanWee/status/1158833601422774273 …pic.twitter.com/A1HuaJA5Kz
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Left is spaghetti plot. Right is ensemble. The ensemble displays variables such as temp and pressure as a way to judge confidence in performance of the models. It's a tool for the forecaster with domain knowledge. Communicating data in science to your own peers is no different.pic.twitter.com/um8rlVwGyD
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Things that have changed since SOLiD is the excellent quality of confocal microscopes, fluorophores and most importantly sCMOS. Dual color base calling would not be possible without that because of highres optical sectioning needed to do 3D base calling.pic.twitter.com/pxNpWHbssS
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We see no star activity. I thought this was going to be a huge issue but only later I found some really nice papers describing inosine and Endo V cleaving in detail and feel less worried about it. Old school results like this: http://www.jbc.org/content/269/50/31390.full.pdf …pic.twitter.com/e2rWQqb5Cc
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That is an really awesome point! This is what the initial thought was when we observed that RNase H2 needed some additional washes to completely remove fluorescence from the amplicon (left) compared to Endo V (right). (1/2)pic.twitter.com/vhGUpe2QlD
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Sure the one marker idea is crazy. But even so Nrn1 is not expressed at sufficient levels in MSNs to be detected by a DiG probe. Just look at the raw data: http://mouse.brain-map.org/experiment/siv?id=75147760&imageId=75169062&initImage=ish&coordSystem=pixel&x=3496&y=2952&z=2 …pic.twitter.com/4hEUDeSf3n
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According to these data the striatum contains a majority of excitatory neurons and oddly enough they seem to only occur at certain single AP coordinate sections...
@BlueBrainPjt@HumanBrainProjpic.twitter.com/fg68TsjyuI
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As a Swedish Jew I’m super happy with this sign my wife made for Friendsgiving potluck we judt went to at Albert Einstein.pic.twitter.com/Zjz3ORTm5Z
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Scaling up imaging of 40x magnification to whole brain scale with smFISH combined with IHC https://www.biorxiv.org/content/early/2018/10/03/432104 … Single-cell in situ transcriptomic map of astrocyte cortical layer diversitypic.twitter.com/2Xy6atbodN
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Looks like HCA Data Coordination Platform use a stock photo of
@Mickan_Asp in their slide deck.#biodata18pic.twitter.com/nXcs99yQ8V
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At
@SfNtweets this afternoon? Interested in basal ganglia and basal forebrain? Go check out 416.18 / DDD13 - Spatial segregation of movement and reward encoding across the basal forebrain cholinergic system. Beautiful data and all new rat atlas by@yoon0632 from@IlanaWitten lab.pic.twitter.com/ulGRLUtMkI -
That moment when that dirty experiment with extremely low probability of working actually turns out to deliver (although more optimization needed) and... it is Friday! So you know that you will start next week with the knowledge of that you are on the right track.pic.twitter.com/8y0k5LPvsB
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Got a quick question on how you do something in WholeBrain? Do as Linda and several others and ask on
@gitchat with your@github login. https://gitter.im/tractatus/Lobby Here is the answer for how you plot only a subset of cells together with the outline (in this case the thalamus).pic.twitter.com/0JgMzSU64q
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Taking the Saturday off from in situ sequencing and spending some much needed time on updating WholeBrain web-interface. Here shown the reslice selection module that will be included in the next update.
#rstatspic.twitter.com/AvQ3dnALAq -
To demonstrate the feasibility of comparing connectomes between model systems Longwen also performed whole isocortex reconstruction of the connectome in a BTBR mouse, an inbred strain lacking the corpus callosum. (4/5)pic.twitter.com/wErTFLOUV5
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Not only does this approach recapture the results of
@AllenInstitute but it also predicts functional connectivity as mapped by@simonMusall@MattAntimatt@anne_churchland (3/5).pic.twitter.com/ECDBwn3ELP
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