Imaging these sections was so rewarding because every single one looks beautiful.pic.twitter.com/vhfiQ1uxwk
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Imaging these sections was so rewarding because every single one looks beautiful.pic.twitter.com/vhfiQ1uxwk
BEAUTIFUL images! Do you mind sharing how/what the tissue was stained with (or how it was otherwise colored)?
Thanks! This is using a pretty standard immunohistochemistry protocol with primary antibodies bound to fluorescent secondary antibodies and then imaged on a Zeiss scope equipped with apotome.
What are your labels?
The 1st and 3rd images are from the same set of markers (but two different brains): paravalbumin + two different antibodies for beta-dystroglycan. The 2nd image is calbindin + calretinin + NPY. The 4th image is parvalbumin + VGluT1 + VGAT. All of them have nuclei labeled in blue.
Can you share a protocol? This is a beauty!!
I can e-mail you my protocol if you'd like - send me a DM!
Nope! These are wild-type brains. Each section is stained with three different antibodies + a nuclear stain (hoechst).
Even did some 'old fashioned' Golgi silver staining on rat cerebellum some years back and counted spines on Purkinje neurites 
One of our ESRs is studying effects of environmental pollutants on developing chicken cerebellum @PROTECTED_ITN @QUB_Disruptor
The complexity of Purkinje arbors is incredible! I bet those Golgi stains looked amazing.
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