What’s the evidence for no effect on samples at -70 ? Have you for example tested what happens to cell extracts if they’re kept at -70 vs -80 for prolonged periods ? Stability of nucleic acids and virus particles at -70 means nothing compared to proteins in cellular extracts !
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Someone else had similar concerns but deleted his tweet and my reply. Here are some more resources for you to browse to help you decide if feasible for your lab: https://scholar.google.com/scholar?q=related:MUzgYwdbppoJ:scholar.google.com/&hl=en&as_sdt=0,7 … ; https://www.i2sl.org/freezerchallenge.html … ; https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/protein-stability …
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Any idea if molecular stability is the same at -70c vs -80c? Was the choice of -80c arbitrary back when people started to use those freezers ? Although saving energy and money is appealing, degrading samples is not... worth a thought maybe ?
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The reference to the scientific evidence backing the assumption that a shift to 70c has no effects on samples is just a website with a simple statement on the linked webpage...
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This is great! Seems like the sort of win-win-win, low hanging fruit that everyone should be looking for- thx for tweeting!
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I am all for being green… In fact my whole farm went solar this summer… But I worry that none of our freezers have a back up power system given our old buildings… So if I raise the temperature on our freezers and the power goes out I have less time until my samples go bad…
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There may not be a universal approach. -70 to conserve energy, -80 to eliminate variables in exp procedures, reg maintenance of the freezer, sample management, etc. All have opportunity for efficiency.
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We do that.
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