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  1. proslijedio/la je Tweet
    7. sij

    First pub of the new year from the lab. "Recording mobile DNA in the gut microbiota using an Escherichia coli CRISPR-Cas spacer acquisition platform" The HGT data is pretty amazing to me even now. Led by & .

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  2. proslijedio/la je Tweet
    6. sij

    Thrilled to share our latest work with the bioinformagician . We measure promoter activity of 500,000+ sequences spanning the entire E. coli genome with 8x coverage and systematically mutate discovered promoters to identify regulatory elements.

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  3. proslijedio/la je Tweet
    4. sij

    New preprint from the lab on a new programmable & portable bacterial CRISPRa system. Not enough of this tech out there. Hope this is useful for folks. Activator screening, directed evolution, transcriptome profiling, promoter libraries.

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  4. proslijedio/la je Tweet
    22. stu 2019.

    Excited to share my work with ! In this preprint, we engineered bacteria to sense and grow in specific physiological cues. We see programmed localization in upper or lower GI tract and reduced off-target colonization.

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  5. proslijedio/la je Tweet
    21. lis 2019.

    Our work dissecting the mechanism of the ZTP Riboswitch is out in ! Congrats to and for incredible work. New roles for strand displacement in RNA mechanisms!

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  6. proslijedio/la je Tweet
    17. ruj 2019.

    Starting a postdoc is so tough. It’s humbling to realize “I have a PhD and I need to ask for help just to find the pipet tips.”

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  7. proslijedio/la je Tweet
    21. kol 2019.

    Our latest paper is out! A model for the spatio-temporal design of gene regulatory circuits - et al.

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  8. proslijedio/la je Tweet
    14. kol 2019.

    Proud to share our new paper on multiplex transcriptional assays in 10 diverse bacterial cell-free TxTl systems in . Lots of Tx/promoter data across phylogeny. Great collab w/ Vincent Noireaux started at course. Led by

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  9. proslijedio/la je Tweet
    8. kol 2019.

    Happy to share our new paper in to design and build synthetic overlapping genes. These "sequence entanglements" (inspired by quantum entanglement) enable more robust genetic systems and a new way for biocontainment.

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  10. proslijedio/la je Tweet
    22. srp 2019.

    happy to share our technique, MaPS-seq, for performing microscopic "plot-sampling" of microbiomes out today in

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  11. proslijedio/la je Tweet
    3. lip 2019.

    New pre-print from our lab: "An Engineered Cas-Transposon System for Programmable and Precise DNA Transpositions". We fused dCas9 with Himar and showed it works as a programmable and targeted integrase.

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  12. proslijedio/la je Tweet
    20. tra 2019.

    My advice for those applying to various new investigator awards along with a couple of my applications from the NIH New Innovator (DP2) and Searle Scholar Awards. [1/6]

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  13. proslijedio/la je Tweet
    12. ožu 2019.

    When infighting distracts us from seeing the big picture: Via

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  14. proslijedio/la je Tweet
    28. velj 2019.

    unfortunate that our students’ submission to the sequencing competition was not chosen! other donors interested in funding these efforts welcome. pls RT.

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  15. proslijedio/la je Tweet
    19. velj 2019.

    Why I believe in cell-free biology by Zachary Sun, an elevator pitch for cell-free bioengineering.

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  16. proslijedio/la je Tweet
    13. velj 2019.

    If we want to make a quick batch of agar plates, departmental autoclave takes >2 hr. We often microwave, which works but is messy. Just ordered Instant Pot Duo 8-qt for the lab. $99 on Amazon, same price as LUX, which has 1 fewer function.

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  17. proslijedio/la je Tweet
    9. velj 2019.

    This really does work for every paper.

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  18. proslijedio/la je Tweet
    1. velj 2019.

    Just in case you've forgotten how routinely breathtaking building a new life is... Here is a stunning reminder:

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  19. proslijedio/la je Tweet
    22. sij 2019.

    balance your centrifuge without using "blanks"

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  20. proslijedio/la je Tweet
    24. sij 2019.

    I like this 'cheat-sheet' of which statistical tests to use when. It's from JGI's IMG toolbox: The paper describing this and other cool tools from IMG:

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