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s_r_constantin's profile
Sarah Constantin
Sarah Constantin
Sarah Constantin
@s_r_constantin

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Sarah Constantin

@s_r_constantin

Math/ML/data-science person now working on solving aging...and helping with COVID19?! Founder, LRI and Daphnia Labs. Married to @oscredwin

Be
srconstantin.posthaven.com
Joined February 2019

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    1. Sarah Constantin‏ @s_r_constantin Mar 13
      • Report Tweet
      • Report NetzDG Violation

      Mammoth Biosciences has developed a CRISPR-based COVID19 test, which can be conducted in 30 min, and is trying to expand to being available at the point of care. https://mammoth.bio/wp-content/uploads/2020/03/Mammoth-Biosciences-A-protocol-for-rapid-detection-of-SARS-CoV-2-using-CRISPR-diagnostics-DETECTR.pdf …

      3 replies 32 retweets 149 likes
      Show this thread
      Sarah Constantin‏ @s_r_constantin Mar 13
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      All of the reagents and devices necessary are listed in the link above and commercially available. The test is portable; you don't need a full lab to conduct it, just portable incubators, pipettes, and somewhere to keep reagents clean + cold.

      1:57 PM - 13 Mar 2020
      • 5 Retweets
      • 22 Likes
      • 🖖 distancing in Melb (Chris Waterguy) @micahstubbs SeaWyrm Joey ae7777 stupid twitt account James Bailey Divia Eden Siberian Fox
      1 reply 5 retweets 22 likes
        1. New conversation
        2. Sarah Constantin‏ @s_r_constantin Mar 13
          • Report Tweet
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          This protocol assumes you already have extracted RNA from the sample. RNA extraction kits are in short supply: there is only one approved manufacturer (Qiagen) though a second (Roche) has recently been approved.https://www.the-scientist.com/news-opinion/rna-extraction-kits-for-covid-19-tests-are-in-short-supply-in-us-67250 …

          1 reply 2 retweets 13 likes
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        3. Sarah Constantin‏ @s_r_constantin Mar 13
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          Even to qualify for the FDA's Emergency Use Authorization requires validation of the entire testing workflow. As I understand, it would be illegal (?) to test patients without an EAU.https://www.modernhealthcare.com/technology/labs-face-challenges-creating-diagnosis-testing-covid-19 …

          1 reply 2 retweets 6 likes
          Show this thread
        4. Sarah Constantin‏ @s_r_constantin Mar 13
          • Report Tweet
          • Report NetzDG Violation

          Sarah Constantin Retweeted Ian Quigley

          So while, as Ian tweeted earlier, you don't need an RNA test kit to do an RNA extraction (https://twitter.com/allmeasures/status/1238089400296202243 …) if you gave patients a COVID-19 test based on the "old-fashioned" phenol/chloroform RNA extraction method, that wouldn't be automatically approved.

          Sarah Constantin added,

          Ian Quigley @allmeasures
          This is truly the finest hour for anti-molecular-biology-kit cranks (I am also a member) https://twitter.com/biochem_bri/status/1238037558921179141 …
          Show this thread
          1 reply 1 retweet 6 likes
          Show this thread
        5. Sarah Constantin‏ @s_r_constantin Mar 13
          • Report Tweet
          • Report NetzDG Violation

          I'm struggling to figure out what would happen, concretely, to a doctor who tested patients with unapproved tests, or a laboratory that provided them. (Fines? Loss of license? what are the penalties here?)

          5 replies 0 retweets 23 likes
          Show this thread
        6. End of conversation

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