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Prikvačeni tweet
A case report of how I debugged a 2P microscope with too low photon yield. The underlying cause turned out to be pulse dispersion at dielectric mirrors, but some other lessons can be learned from that as well. https://ptrrupprecht.github.io/Photon-yield-and-pulse-dispersion/ …pic.twitter.com/6QC5E4o1kt
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Peter Rupprecht proslijedio/la je Tweet
To celebrate 1k followers, here's the first post in a series where I review various glues. We start with the 5/5 star rated top entry: epoxy. http://jvoigts.scripts.mit.edu/blog/review-epoxy/ …
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Peter Rupprecht proslijedio/la je Tweet
1/8 Excited to announce that our paper is out in eLife: https://elifesciences.org/articles/49145 Thank you
@ValerioFrancio1 and@ZPadamsey for the hard work! Special thanks to eLife for their amazing editorial process. Wish all journals would adopt similar policies.Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Cool to see this huge paper finally out! 2P imaging plus dense EM reconstruction of the entire brain region in the same animal - congrats to
@AdrianAWanner !https://twitter.com/AdrianAWanner/status/1219294228288811008 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Peter Rupprecht proslijedio/la je Tweet
Very happy to share this preprint from my main PhD project. We performed wide-field calcium imaging on animals solving both auditory and tactile discrimination tasks with a delay. Many thanks to
@FritjofHelmchen,@ArielGilad , Balazs Laurenczy and Fabian Voigt@MesoSpim.https://twitter.com/FritjofHelmchen/status/1217462044775063552 …
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Peter Rupprecht proslijedio/la je Tweet
Fascinated by
#lightsheet#Microscopy? Curious about tissue clearing? We're hiring - the#mesoSPIM team in the lab of@FritjofHelmchen@UZH_en is looking for a talented PhD student - more info: http://bit.ly/2NoxRSU - please RT!pic.twitter.com/mRRI4VK18I
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Peter Rupprecht proslijedio/la je Tweet
The Friedrich Lab at the Friedrich Miescher Institute (
@FMIscience) in Basel, Switzerland, is seeking a scientist to support advanced experimental applications and data analysis for Zebrafish neuroscience: https://www.fmi.ch/education-careers/positions/jobdetails.html?jobID=257 …#academia#jobsearchHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
The behavior of single-cell organisms can be described by specific goals, their objective function. Neurons are also single cells. Does it make sense to think about the objective function of a single neuron? Blog post inspired by the work of
@RomainBrette: https://ptrrupprecht.wordpress.com/2019/12/31/annual-report-of-my-intuition-about-the-brain-2019/ …pic.twitter.com/e5kHqeIZ2n
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Peter Rupprecht proslijedio/la je Tweet
Incidentally, exaggeration~4 seems to correspond pretty well to the default UMAP settings. Here shown for MNIST, but I have observed the same on many other data sets too. (14/16)pic.twitter.com/66AgY2gOIy
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Best video instructions on how to align a 2P microscope that I've seen so far, directly from the bench! (Plus, more videos on adaptive optics and a demonstration of fourier optics.)https://twitter.com/NemonicHub/status/1202364885402710016 …
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Peter Rupprecht proslijedio/la je Tweet
My first paper on Paramecium, the swimming neuron! "A simple device to immobilize protists for electrophysiology and microinjection" https://www.biorxiv.org/content/10.1101/859413v1 … All movies on my web site:http://romainbrette.fr/neuroscience-of-a-swimming-neuron/ …
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Peter Rupprecht proslijedio/la je Tweet
Too much Turkey? Check out /r/neuroscience today for an AMA with Bobby Kasthuri and me about EM connectomics.https://www.reddit.com/r/neuroscience/comments/e2x2bc/we_are_j%C3%B6rgen_kornfeld_and_bobby_kasthuri_and/ …
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Very cool and systematic ground truth recordings with cell-attached and simultaneous calcium imaging for 91 neurons. Actually, it's rather 237, but the other neurons were discarded by quality control!https://twitter.com/biorxiv_neursci/status/1179181922771226624 …
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Peter Rupprecht proslijedio/la je Tweet
Spatiotemporal refinement of signal flow through association cortex during learninghttps://www.biorxiv.org/content/10.1101/732115v1 …
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Paper 2 (preprint): Francioni and Rochefort, High somato-dendritic coupling of V1 layer 5 neurons independent of behavioural state and visual stimulation https://www.biorxiv.org/content/10.1101/664177v2 …
@RochefortLabPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Paper 1: Beaulieu-Laroche, Harnett et al., Widespread and highly correlated somato-dendritic activity in cortical layer 5 neurons https://www.cell.com/neuron/fulltext/S0896-6273(19)30439-8 …
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Two recent papers address an important dendrite question using calcium imaging: How is activity in the soma and the apical dendrite coupled for L5 neurons? The short answer is: very tightly. I wrote up a summary of these studies on my blog:https://ptrrupprecht.wordpress.com/2019/07/10/post-publication-review-somato-dendritic-coupling-of-l5-neurons-in-v1/ …
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Peter Rupprecht proslijedio/la je Tweet
I am very excited to share our study where we compared how L2/3 and L5 neurons integrate locomotion with whisker touch.
@Aman_sal@FritjofHelmchen. Do not forget to check supplementary information, it is rich. https://rdcu.be/bGALG pic.twitter.com/Ovf2zC74nT
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Peter Rupprecht proslijedio/la je Tweet
Microscopy technique development's exploding, but core facilities still buy confocals. This ugly baby will end that. Less phototoxicity, WAY faster than confocal. NO DRAWBACK. Details here: https://andrewgyork.github.io/high_na_single_objective_lightsheet/ … Co-invented by
@amsikking. Get one from jon@asiimaging.compic.twitter.com/vFs5KKAxij
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Peter Rupprecht proslijedio/la je Tweet
From the
@FritjofHelmchen lab: High-density arrays of optical fibers for monitoring and manipulating neural activity at large scale across many brain regions. https://www.nature.com/articles/s41592-019-0400-4 …pic.twitter.com/mIuQLxM25S
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That is a very clear and interesting discussion of an artefact in high-pass filtered LFP recordings! I wouldn't have thought that something which looks as much as a biological effect like this might be an artefact. Now I know better.https://twitter.com/michael_okun/status/1127205543645466624 …
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