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Back to back read until preprints on
@biorxivpreprint on@nanopore#readuntilhttps://twitter.com/mattloose/status/1224350276083240960 …
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Olivier proslijedio/la je Tweet
Read Until to enrich >700 genes to mean 30x coverage on
@nanopore single flowcell. Custom panels (up to 25,600 targets so far) as easy as providing a coordinate file. Read more at https://www.biorxiv.org/content/10.1101/2020.02.03.926956v1 … - thanks to@alexomics,@Rorymatics and@DeepSeqNotts team.pic.twitter.com/0FBLlGQZdm
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Olivier proslijedio/la je Tweet
Introducing "Targeted nanopore sequencing by real-time mapping of raw electrical signal with UNCALLED" with
@samkovaka@timp0 et al. Does selective enrichment and depletion of any targeted genomic region purely in software for@nanopore sequencing.https://www.biorxiv.org/content/10.1101/2020.02.03.931923v1 …Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Dawn of a new era for targeted sequencing (
#exome#genomics) : no enrichment prep, focus on enriching information during the run, bioinformatically. Only possible with@nanoporehttps://twitter.com/mike_schatz/status/1224364244293971968 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Si la communauté française
@nanopore sur Twitter souhaite se manifester et/ou commenter.https://twitter.com/Billaut/status/1223463044531003393 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Olivier proslijedio/la je Tweet
First release of pycoMeth for
@nanopore genome-wide differential DNA methylation analysis from@jaredtsimpson Nanopolish call-methylation. Generates summary for CpG dinucleotides and CpG islands. Basic visualisation with genome Brower, ie#IGV. https://github.com/a-slide/pycoMeth …pic.twitter.com/xCDvuQE6fl
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Olivier proslijedio/la je Tweet
Our educational guide for nanopore sequencing in the classroom is out in
@PLOSCompBiol. Introducing bioinformatics students to the realities of data acquisition, an educational collaboration of@StanBrouns at@TNWTUDelft and myself at@EEMCS_TUD.https://journals.plos.org/ploscompbiol/article?id=10.1371/journal.pcbi.1007314 …Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Olivier proslijedio/la je TweetHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Olivier proslijedio/la je Tweet
We sequenced a brain sample on
@nanopore PromethION. So far 107Gb with an N50 of 36.31Kb has been sequenced. Thanks to some great applications from@circulomics and@Diagenode ! Will refuel one more time.@wouter_decoster@MojcaStrazisar@VIBTechWatchpic.twitter.com/f2ICf04Adv
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Olivier proslijedio/la je Tweet
We’ve got primer pools ready to go for a 98-plex scheme which span nCoV-2019. Get in touch if you want an aliquot
pic.twitter.com/qvHNgYS8Hx
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Olivier proslijedio/la je Tweet
ARTIC have developed a set of lab and bioinformatics protocols for the nCoV-2019 virus using a targeted multiplex primer scheme. This package enables direct sequencing and real-time analysis with RAMPART of the coronavirus on nanopore sequencers: https://artic.network/ncov-2019 pic.twitter.com/hPbvmpX5DE
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Olivier proslijedio/la je Tweet
Sequencing ~120mb rotifer genome using
@nanopore#MinION MK1C in Advanced Molecular Ecology course@wur. In real-real time. Students isolated DNA with@ZymoResearch HMW magbead kit, checked with@miniPCR#Bluegel, prepared LSK109 library. Read N50 >30kb, >2.3Gb in 4h, ongoing.pic.twitter.com/IFm2apx7cr
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Olivier proslijedio/la je Tweet
Anti-GMO folks: you've been drinking GMO beer and tea for your whole life. Want to reconsider your unscientific opposition to all GMOs?https://www.forbes.com/sites/stevensalzberg/2020/01/20/surprise-here-are-12-organic-foods-that-are-transgenic-gmos/ …
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Olivier proslijedio/la je Tweet
I am really excited to finally share our SHARC project! Rapid and cheap low coverage
@nanopore sequencing of tumor genomes to discover tumor-specific biomarkers that allow tracking the tumor in blood. https://www.medrxiv.org/content/10.1101/19011932v1 … 1/6Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Olivier proslijedio/la je Tweet
On optimal lengths in long read sequencing https://doi.org/10.1093/nargab/lqz027 …
@wouter_decoster@t_depooter@PacBio@nanoporeHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Optimal read length to discover SV (human) is at least 20kb and 100kb is optimal for haplotyping as per https://academic.oup.com/nargab/article/2/1/lqz027/5701459 …https://twitter.com/wouter_decoster/status/1217006112202948608 …
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Olivier proslijedio/la je Tweet
From the microbes of Iceland’s polar icecaps, to meningitis ID in Vietnam. From rapid cancer characterisation to food safety. From 1000s of human genomes, to the tallest trees in the world – we'd like to share our 19 highlights of 2019: http://bit.ly/2F932gl pic.twitter.com/WcgvNyCtAn
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Olivier proslijedio/la je TweetHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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105 trees/seedlings/pits planted. 1/3 of my 2019
#carbonfootprint#CarbonOffsetInitiative done. Should be able to do 150 more in Jan and be close.Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Olivier proslijedio/la je Tweet
We like
#single-molecules, all things#RNA and#archaea! Here is a new twist on this: phantastic work by@FelixGrunberger who performed native RNA sequencing using the#Nanopore sequencing platform to characterise bacterial and archaeal transcriptomes!https://www.biorxiv.org/content/10.1101/2019.12.18.880849v1 …Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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