Olivier

@olivierlucas29

Former scientist, forever in love with Science, Travel and Oceans. employee. Opinions are all my own.

Vrijeme pridruživanja: rujan 2011.

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  1. prije 6 sati
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    prije 7 sati

    Read Until to enrich >700 genes to mean 30x coverage on single flowcell. Custom panels (up to 25,600 targets so far) as easy as providing a coordinate file. Read more at - thanks to , and team.

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  3. proslijedio/la je Tweet
    prije 6 sati

    Introducing "Targeted nanopore sequencing by real-time mapping of raw electrical signal with UNCALLED" with et al. Does selective enrichment and depletion of any targeted genomic region purely in software for sequencing.

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  4. prije 6 sati

    Dawn of a new era for targeted sequencing ( ) : no enrichment prep, focus on enriching information during the run, bioinformatically. Only possible with

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  5. 1. velj

    Si la communauté française sur Twitter souhaite se manifester et/ou commenter.

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  6. proslijedio/la je Tweet
    28. sij

    First release of pycoMeth for genome-wide differential DNA methylation analysis from Nanopolish call-methylation. Generates summary for CpG dinucleotides and CpG islands. Basic visualisation with genome Brower, ie .

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  7. proslijedio/la je Tweet
    28. sij

    Our educational guide for nanopore sequencing in the classroom is out in . Introducing bioinformatics students to the realities of data acquisition, an educational collaboration of at and myself at .

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    24. sij
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  9. proslijedio/la je Tweet
    24. sij

    We sequenced a brain sample on PromethION. So far 107Gb with an N50 of 36.31Kb has been sequenced. Thanks to some great applications from and ! Will refuel one more time.

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  10. proslijedio/la je Tweet
    24. sij
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    We’ve got primer pools ready to go for a 98-plex scheme which span nCoV-2019. Get in touch if you want an aliquot 🙋‍♂️

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  11. proslijedio/la je Tweet
    24. sij

    ARTIC have developed a set of lab and bioinformatics protocols for the nCoV-2019 virus using a targeted multiplex primer scheme. This package enables direct sequencing and real-time analysis with RAMPART of the coronavirus on nanopore sequencers:

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  12. proslijedio/la je Tweet
    22. sij

    Sequencing ~120mb rotifer genome using MK1C in Advanced Molecular Ecology course . In real-real time. Students isolated DNA with HMW magbead kit, checked with , prepared LSK109 library. Read N50 >30kb, >2.3Gb in 4h, ongoing.

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  13. proslijedio/la je Tweet
    20. sij

    Anti-GMO folks: you've been drinking GMO beer and tea for your whole life. Want to reconsider your unscientific opposition to all GMOs?

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    I am really excited to finally share our SHARC project! Rapid and cheap low coverage sequencing of tumor genomes to discover tumor-specific biomarkers that allow tracking the tumor in blood. 1/6

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  15. proslijedio/la je Tweet
    14. sij
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  16. 14. sij

    Optimal read length to discover SV (human) is at least 20kb and 100kb is optimal for haplotyping as per

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    From the microbes of Iceland’s polar icecaps, to meningitis ID in Vietnam. From rapid cancer characterisation to food safety. From 1000s of human genomes, to the tallest trees in the world – we'd like to share our 19 highlights of 2019:

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  18. proslijedio/la je Tweet
    28. pro 2019.
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  19. 23. pro 2019.

    105 trees/seedlings/pits planted. 1/3 of my 2019 done. Should be able to do 150 more in Jan and be close.

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  20. proslijedio/la je Tweet
    20. pro 2019.

    We like -molecules, all things and ! Here is a new twist on this: phantastic work by who performed native RNA sequencing using the sequencing platform to characterise bacterial and archaeal transcriptomes!

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