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Prikvačeni tweet
Towards real-time targeting/enrichment/other sampling on the
@nanopore sequencer, not in the sample prep: two new preprints from Kovaka/Payne show adaptive sampling using ‘Read Until’ API, find out more here: https://nanoporetech.com/about-us/news/towards-real-time-targeting-enrichment-or-other-sampling-nanopore-sequencing-devices … 1/3pic.twitter.com/jHvb8TqvUi
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Oxford Nanopore proslijedio/la je Tweet
Yeah - you simply reverse the voltage over the specific pore.https://www.nature.com/articles/nmeth.3930 …
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Oxford Nanopore proslijedio/la je Tweet
Early testing of rat whole chromosome “selection”. Different surface zones on a single flowcell used for different Chrm selection/rejection. (Median read lengths per chromosome)
. Looking forward to some finer grain target region stuff on a basic GPU enabled desktop/laptop!pic.twitter.com/N2NzECdfmS
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Oxford Nanopore proslijedio/la je Tweet
And thanks to
@generoom for hooking us up with some cell lines to help show that we can detect a PML-RARA fusion in a tumour cell line with only 15 hours sequencing using the https://cancer.sanger.ac.uk/cosmic cancer panel (717 genes). https://www.biorxiv.org/content/10.1101/2020.02.03.926956v1 …pic.twitter.com/wBU6KsaiJt
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Oxford Nanopore proslijedio/la je Tweet
.
@nanopore's write up on how adaptive sequencing works by controlling pore voltages in real time. Amazing that we can have so much control over individual molecules of DNA! https://nanoporetech.com/about-us/news/towards-real-time-targeting-enrichment-or-other-sampling-nanopore-sequencing-devices …@samkovaka@mattloose@timp0@alexomics#StarTrekpic.twitter.com/CoDD9kAyE9
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Oxford Nanopore proslijedio/la je Tweet
Yes, coverage over target region has little to no bias and follows a Poisson distribution exactly as expected.pic.twitter.com/zigUgKxOU9
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Oxford Nanopore proslijedio/la je TweetHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Oxford Nanopore proslijedio/la je Tweet
Introducing "Targeted nanopore sequencing by real-time mapping of raw electrical signal with UNCALLED" with
@samkovaka@timp0 et al. Does selective enrichment and depletion of any targeted genomic region purely in software for@nanopore sequencing.https://www.biorxiv.org/content/10.1101/2020.02.03.931923v1 …Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Oxford Nanopore proslijedio/la je Tweet
Read Until to enrich >700 genes to mean 30x coverage on
@nanopore single flowcell. Custom panels (up to 25,600 targets so far) as easy as providing a coordinate file. Read more at https://www.biorxiv.org/content/10.1101/2020.02.03.926956v1 … - thanks to@alexomics,@Rorymatics and@DeepSeqNotts team.pic.twitter.com/0FBLlGQZdm
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Payne et al. target a human exome panel covering over 10,000 genes, enrich from a mixed sample, COSMIC 717-gene panel including fusion event, using adaptive sampling
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Kovaka et al. enrich for 148 human genes associated with hereditary cancers to 30X coverage on a single MinION flow cell
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Reminder! Vania Costa's knowledge exchange, titled 'a primer on nucleic acid extraction', will be taking place tomorrow @ 3pm in the Community! Find out more here: http://bit.ly/2tE6D47 (login required)pic.twitter.com/Gx2QIcHECq
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Oxford Nanopore proslijedio/la je Tweet
Our paper using
@nanopore sequencing for rapid in-country genomic surveillance of#rabies virus has just been published@WellcomeOpenRes! This was a huge effort from many people across 4 countries

over 3years, thanks to all involved! Please RT! https://wellcomeopenresearch.org/articles/5-3/v1 pic.twitter.com/K6P6CYZW0w
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Oxford Nanopore proslijedio/la je Tweet
Siadjeu et al. providing insights into the yam genome using
@nanopore reads. The assembly consisted of 35,269 protein-encoding genes with a total length of 485 Mbp. bioRxiv viewerhttp://disq.us/t/3ltb8axHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Oxford Nanopore proslijedio/la je Tweet
Very fruitful collaboration between
@AlbachLab@UniOldenburg and@CeBiTec:@nanopore sequencing of yam DNA, assembly of genome sequence & annotation. About 6 months from first meeting with@siadjeu2012 to#preprint@bioRxiv.https://bit.ly/2UfWLZbPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Oxford Nanopore proslijedio/la je Tweet
'High contiguity de novo genome sequence assembly of Trifoliate yam (Dioscorea dumetorum) using long read sequencing'
#plantsci#genomics#bioinformatics https://doi.org/10.1101/2020.01.31.928630 …pic.twitter.com/7mEAiWSTHs
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Oxford Nanopore proslijedio/la je Tweet
Totally fascinated by the DNA tech from
@nanopore ! Wish we had it for our expeditions to French Guiana and Polynesia ! I loved filming DNA seq in a Montenegro cave with@mdelledon & team#expedition#dnasequencing#oxfordnanopore#taxonexpeditionshttps://www.youtube.com/watch?v=4E68tD295gY …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
A Nanopore Day is taking place in New Delhi, India on 19th February! Check out the full agenda and apply for a place here: http://bit.ly/2TYL74I pic.twitter.com/momX9ZeLP2
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Oxford Nanopore proslijedio/la je Tweet
Multitasking on the GridION. Flongles working alongside the MinION flowcells! Crazy how small the Flongles are.
#science#minION#flongle#gridION#molecularbiology#dna#dnasequencing#geek#nerd#urbangeek#urbanscientist#urbannerd#nanopore#ont#oxfordnanoporepic.twitter.com/fsMuDojlUt
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Oxford Nanopore proslijedio/la je Tweet
We scanned the
@PHE_uk Salmonella WGS collection for mobile colistin resistance genes and used@nanopore to describe four novel MCR encoding plasmids and several chromosomal integration events@MicrobioSoc https://doi.org/10.1099/mgen.0.000331 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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