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We can have all of the test kits we need, but they rely on our ability to get the RNA out of a sample first, for testing. As this epidemic becomes in full swing, many additional reagents or parts that we never usually question the availability of may become difficult to find.
Anyway to get them from China?
Many of the reagents are made in china already. With slow down in production there, it is likely at least in part why we are seeing supplies dwindle here. Or, perhaps China and S. Korea purchased them all while the US was not acting to get testing set up quickly enough.
Could always do an old fashioned ethanol precipitation method, sure kits make at a bit faster, but we always did the old method since it was massively cheaper.
No, you can't switch extraction methods in a clinical assay, you'd have to revalidate sensitivity/specificity etc.
We already have a mask shortage. I don't want my staff collecting samples without safety gear.
What happened to the 30 million stock of N95？
I assume that the samples are collected into a lysis buffer and there are wash buffers and columns in the kit.
The case definition will change to clinical confirmation rather than lab-confirmed once numbers get big enough.
Here's the quintessential old-school RNA extraction method via acid-phenol-chloroform (basically where the Trizol kit comes from): 1. Chomczynski + Sacchi / Nature Protocols (2006) https://www.ncbi.nlm.nih.gov/m/pubmed/17406285/ … 2. Sambrook (old-school molecular cloning book): Ch.7....
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