Michael Cianfrocco

@mcianfro

Assistant Professor & Dept. of Biological Chemistry / / / / . pronouns: he/him/his

Ann Arbor, MI
Vrijeme pridruživanja: travanj 2012.

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  1. Prikvačeni tweet
    21. pro 2019.

    Excited to share a new preprint from the lab: "High-throughput cryo-EM enabled by user-free preprocessing routines"! TL;DR - we created an automated preprocessing pipeline (1/n)

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  2. prije 21 sat

    Should be another great workshop! Join us in Ann Arbor this June !

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  3. proslijedio/la je Tweet
    31. sij

    I'm really getting stoked about this podcast. Interviews with early career scientist about cutting edge research in . Two interviews recorded, working on the audio. Here's the intro!

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  4. 31. sij

    For the AAA+ nerds out there - such a cool structure! AAA+ GTPase 🤯

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  5. 31. sij
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  6. 28. sij

    Campus networking is very important in - glad to be a part of this effort by !

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  7. proslijedio/la je Tweet
    23. sij

    “The inflated ego that comes with success — the bigger salary, the nicer office, the easy laughs — often makes us feel as if we’ve found the eternal answer to being a leader. But the reality is, we haven’t.“/1

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  8. 22. sij

    2: you can have a lot of aberrations in your dataset and still get good 2D class averages. So- use a lot of beam-image shift for fast data collection and don't touch the microscope if your screening all day!

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  9. 22. sij

    Take home messages 1: microscope aberration correction can really overcome microscope alignment issues!

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  10. 22. sij

    These beam-image shifts definitely introduced aberrations. We could only get a structure of aldolase to 5.6Å initially (!). But, after repeated CTF refinement and particle polishing in , we could increase the resolution from 5.6Å to 2.8Å. Very impressive !

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  11. 22. sij

    These big beam-image shifts definitely caused some significant aberrations on the micrographs. In some cases, it caused serious objective astigmatism (right)

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  12. 22. sij

    We collected over a 10um x 10um area giving us a wide range of images with different beam-image shifts

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  13. 22. sij

    So! set to it - collecting an aldolase dataset on our Talos-Arctica. A part of this work was that we did not do any microscope alignments day-of, simulating what a 'worse case' scenario could be for the instrument.

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  14. 22. sij

    This came from me asking many people: what is the limit for using beam-image shift to collect data at 200 keV? Everyone said it should be worse at 200 keV than 300 keV but we were surprised no one had benchmarked this yet

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  15. 22. sij

    New preprint from the lab: "High-resolution cryo-EM using beam-image shift at 200 keV." Take home message: even with a pretty poorly aligned instrument, you can overcome these aberrations computationally with (1/n)

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  16. proslijedio/la je Tweet

    The US government is weighing a policy that would make the results of US government funded research immediately freely available to everyone. But it's facing fierce opposition. Show your support for by adding your name to this open letter.

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  17. proslijedio/la je Tweet
    22. sij

    Patients and scientists: THIS IS NOT A DRILL. The White House is considering requiring open access to all taxpayer-funded research. Please sign to let The White House know that you strongly support this transformational initiative:

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  18. 19. sij

    After ironing out some issues on our end - we are happy to share the neural network files & installation instructions for MicAssess & 2DAssess. Enjoy!

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  19. proslijedio/la je Tweet
    11. sij

    Attention all Molecular Motors fans! Check out the program for the Cytoskeletal Motors GRC this July. Additional talks will be chosen from submitted abstracts. Apply early because this meeting is typically oversubscribed.

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  20. proslijedio/la je Tweet
    11. sij

    Let's start off the posts with this animation I made. It's a parody of a Microsoft surface product ad.

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  21. proslijedio/la je Tweet
    9. sij

    Agreed! 📷 by @forystrentals: The view of an icy-blue Lake Michigan next to snowy dunes. Does winter get better than this? ⁠

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