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Prikvačeni tweet
Excited to share a new preprint from the lab: "High-throughput cryo-EM enabled by user-free preprocessing routines"! TL;DR - we created an automated
#cryoEM preprocessing pipeline (1/n) https://www.biorxiv.org/content/10.1101/2019.12.20.885541v1 …pic.twitter.com/X1VU3hoWoH
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Should be another great workshop! Join us in Ann Arbor this June !https://twitter.com/umlifesciences/status/1224734476560814080 …
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Michael Cianfrocco proslijedio/la je Tweet
I'm really getting stoked about this
#cryo2go podcast. Interviews with early career scientist about cutting edge research in#cryoEM. Two interviews recorded, working on the audio. Here's the intro!https://open.spotify.com/show/7pU4K9q31s3lnQWTMt7nRm?si=TzHg_zvcQrqBNYhCV4iLig …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
For the AAA+ nerds out there - such a cool structure! AAA+ GTPase
https://twitter.com/cryoEM_Papers/status/1223214439509323777 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
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Campus networking is very important in
#cryoEM - glad to be a part of this effort by@ARCTS_UM! https://twitter.com/UMLifeSciences/status/1222195128124637185 …pic.twitter.com/Dff5Yx9YFmHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Michael Cianfrocco proslijedio/la je Tweet
“The inflated ego that comes with success — the bigger salary, the nicer office, the easy laughs — often makes us feel as if we’ve found the eternal answer to being a leader. But the reality is, we haven’t.“/1https://hbr.org/2018/11/ego-is-the-enemy-of-good-leadership?utm_source=facebook&utm_medium=social&utm_campaign=hbr …
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2: you can have a lot of aberrations in your dataset and still get good 2D class averages. So- use a lot of beam-image shift for fast data collection and don't touch the microscope if your screening all day!pic.twitter.com/BzXJsnvhuZ
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Take home messages 1: microscope aberration correction can really overcome microscope alignment issues!pic.twitter.com/Vq03dYoMSQ
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These beam-image shifts definitely introduced aberrations. We could only get a structure of aldolase to 5.6Å initially (!). But, after repeated CTF refinement and particle polishing in
#RELION, we could increase the resolution from 5.6Å to 2.8Å. Very impressive#RELION!pic.twitter.com/wYiCoxSOc6
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These big beam-image shifts definitely caused some significant aberrations on the micrographs. In some cases, it caused serious objective astigmatism (right)pic.twitter.com/N8kkFEiNpr
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We collected over a 10um x 10um area giving us a wide range of images with different beam-image shiftspic.twitter.com/nVU67H2cqz
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So!
@annotated_sci set to it - collecting an aldolase dataset on our Talos-Arctica. A part of this work was that we did not do any microscope alignments day-of, simulating what a 'worse case' scenario could be for the instrument.Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
This came from me asking many people: what is the limit for using beam-image shift to collect data at 200 keV? Everyone said it should be worse at 200 keV than 300 keV but we were surprised no one had benchmarked this yet
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New preprint from the lab: "High-resolution cryo-EM using beam-image shift at 200 keV." Take home message: even with a pretty poorly aligned
#cryoEM instrument, you can overcome these aberrations computationally with#RELION (1/n)https://www.biorxiv.org/content/10.1101/2020.01.21.914507v1 …Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Michael Cianfrocco proslijedio/la je Tweet
The US government is weighing a policy that would make the results of US government funded research immediately freely available to everyone. But it's facing fierce opposition. Show your support for
#OAinTheUSA by adding your name to this open letter. http://oaintheusa.comHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Michael Cianfrocco proslijedio/la je Tweet
Patients and scientists: THIS IS NOT A DRILL. The White House is considering requiring open access to all taxpayer-funded research. Please sign to let The White House know that you strongly support this transformational initiative: http://www.oaintheusa.com/
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After ironing out some issues on our end - we are happy to share the neural network files & installation instructions for MicAssess & 2DAssess. Enjoy!
#cryoEM https://github.com/cianfrocco-lab/Automatic-cryoEM-preprocessing …https://twitter.com/mcianfro/status/1208450336274432004 …
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Michael Cianfrocco proslijedio/la je Tweet
Attention all Molecular Motors fans! Check out the program for the Cytoskeletal Motors GRC this July. Additional talks will be chosen from submitted abstracts. Apply early because this meeting is typically oversubscribed. https://www.grc.org/cytoskeletal-motors-conference/2020/ …
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Michael Cianfrocco proslijedio/la je Tweet
Let's start off the
#microtubule posts with this animation I made. It's a parody of a Microsoft surface product ad. https://youtu.be/JBYRBbjj3Kk#sciartHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Michael Cianfrocco proslijedio/la je Tweet
Agreed!
by @forystrentals: The view of an icy-blue Lake Michigan next to snowy dunes. Does winter get better than this? pic.twitter.com/LePclQeJFc
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