José Ignacio Rojas Echenique

@jireva

Evolutionary biologist. Postdoc with Charlie Boone and Brenda Andrews at the University of Toronto.

Toronto, ON
Vrijeme pridruživanja: siječanj 2010.

Medijski sadržaj

  1. and I designed an in vitro assay to measure the stability of Cre-Lox integration products and tested a large number of inverted repeat mutations from the literature.

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  2. If the product of recombination contains a doubly mutated Lox site, the reaction equilibrium shifts towards integration.

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  3. An ingenious solution to this problem takes advantage of mutant Lox inverted repeats (IRs) thought to decrease Cre binding. Since Cre binding to Lox IRs is cooperative, a site with a single mutated IR will recombine normally but a double mutant site will not.

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  4. Our system requires that the barcodes we integrate into the genome stay there,even after hundreds of generations of evolution. Unfortunately, Cre-Lox integration is reversible. Worse yet, at equilibrium, integration substrates are favored over integrated product.

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  5. I wish graphviz could draw this pretty

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