-
snRNASeq from frozen tissue (any tissue!) working like a charm! Sweat and tears but finally nailed it! "The devil is in the detail". Happy to help if you are having troubles.
#snRNASeq#10xGenomics@10xgenomics -
I’m struggling with a
#zebrafish#snRNAseq CellRanger alignment. Does anyone have a premrna reference build? Please RT/send help

-
When ‘Frankenstein’ outperforms itself using 3’ v3 for
#snRNAseq. Those UMI counts are insane!
@10xGenomicspic.twitter.com/Xl7ouiKGFP
-
Trying to determine how changes in key genes in specific cell types in the brain contribute to diseases including
#ALS and/or#FTD? But your#snRNAseq is just too noisy? Check out SCINET http://ow.ly/ocL330obpb3@manoliskellis@biorxivpreprint via@AmmarAlChalabipic.twitter.com/b1Us8GKZ6Y
-
Scanpy is an extendable Python based
#snRNASeq analysis package - thx for the interest! Link: http://github.com/theislab/scanpy https://twitter.com/torstenseemann/status/1002834035507253249 … -
@viegasf &@wattenberg mentioned PCA, UMAP and TSNE methods for visualizing high dimensional data at their#rstudioconf keynote We use them also in#genomics like in#scRNAseq#snRNAseq#SpatialTranscriptomics It’s great to see the overlap in methods across fields ^^pic.twitter.com/rIFvwogjQJ
Prikaži ovu nit -
Another question for Dr. Zhang at
#singlecellgenomicsday: for difficult tissues to dissociate to single cells (like brain tissue), does#snRNAseq actually yield useful/less noisy data from more cells than#scRNAseq?@SatijaLabPrikaži ovu nit -
Question for Dr. Zhang at
#singlecellgenomicsday: is#snRNAseq better for approaches that actually require intronic reads, such as RNA velocity? -
@TheSurajKannan does it makes sense in the context of polynucleated cells to do#snRNAseq? Can the heterogeneity seen in this context be an artefact of looking at individual nuclei potentially from the same#singlecell? -
2nd week of
#scQA Forum for#singlecell sample preparation!@LGMartelotto made it easy this week:#scRNAseq vs.#snRNAseq Please join by contributing your experience/question for a lively discussion! https://twitter.com/LGMartelotto/status/1216546501142278144 … -
#scQC Jan13/2020#singlecell Question and Answer Forum (w/@hoheyn and@LGMartelotto). THIS WEEK:#snRNAseq vs.#scRNAseq -scRNAseq methods are optimal when cells can be harvested intact and viable.Prikaži ovu nit -
Single cell symposium
@DanaFarber Pros and cons of single nucleus RNA-seq from frozen tissues ____#scRNAseq#cancer#symposium#AcademicTwitter#snRNAseq pic.twitter.com/2zWHwFZ8AK
-
Works like a charm. We’re really enjoying
#scRNAseq#snRNAseq@fluidigm The possibilities are endless.



https://twitter.com/GLim2016/status/1192976919517683712 … -
Next,
#scDblFinder by Pierre-Luc Germain which builds upon#SingleCellExperiment (sce) and finds doublets with#scRNAseq data (or#snRNAseq like we have) https://bioconductor.org/packages/release/bioc/vignettes/scDblFinder/inst/doc/scDblFinder.html …pic.twitter.com/BMoO1ObAB6
Prikaži ovu nit -
Stereotyped transcriptomic transformation of somatosensory neurons in response to injury |
@eLife#neuroscience#scRNAseq#snRNAseq https://elifesciences.org/articles/49679 -
DON’T FORGET to register (link below) to the second SCRUM! Room is filling up quickly!!! ZOOM will be available!
@UMCCR@PeterMacRes@WEHI_research@ONJCRI@ARMI_Labs@MCRI_for_kids@latrobe@GarvanInstitute also, sign up to get updates of coming events.!#SingleCell#snRNASeq https://twitter.com/LGMartelotto/status/1158974474386997249 … -
Happy to share my first preprint on subthalamic nucleus!
#snRNAseq#STNhttps://twitter.com/biorxiv_neursci/status/1141753836895428608 … -
Very good question....I also would like to store nuclei from FACS...
#snrnaseq#10x#PhDForumhttps://twitter.com/achamess/status/1131966255253131265 … -
Up now! Eugene Drokhlyansky on human single-cell atlas using single-nucleus RNA-Seq
#snRNASeq#10xugm19pic.twitter.com/SiQH99IepN
Prikaži ovu nit
Čini se da učitavanje traje već neko vrijeme.
Twitter je možda preopterećen ili ima kratkotrajnih poteškoća u radu. Pokušajte ponovno ili potražite dodatne informacije u odjeljku Status Twittera.