Rezultati pretraživanja
  1. snRNASeq from frozen tissue (any tissue!) working like a charm! Sweat and tears but finally nailed it! "The devil is in the detail". Happy to help if you are having troubles.

  2. 2. kol 2019.

    I’m struggling with a CellRanger alignment. Does anyone have a premrna reference build? Please RT/send help 🙏🐟

  3. When ‘Frankenstein’ outperforms itself using 3’ v3 for . Those UMI counts are insane! 😌

  4. 25. ožu 2019.

    Trying to determine how changes in key genes in specific cell types in the brain contribute to diseases including and/or ? But your is just too noisy? Check out SCINET via

  5. 2. lip 2018.

    Scanpy is an extendable Python based analysis package - thx for the interest! Link:

  6. & mentioned PCA, UMAP and TSNE methods for visualizing high dimensional data at their keynote We use them also in like in It’s great to see the overlap in methods across fields ^^

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  7. 29. sij
  8. Another question for Dr. Zhang at : for difficult tissues to dissociate to single cells (like brain tissue), does actually yield useful/less noisy data from more cells than ?

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  9. Question for Dr. Zhang at : is better for approaches that actually require intronic reads, such as RNA velocity?

  10. 13. sij
    Odgovor korisniku/ci

    does it makes sense in the context of polynucleated cells to do ? Can the heterogeneity seen in this context be an artefact of looking at individual nuclei potentially from the same ?

  11. 13. sij

    2nd week of Forum for sample preparation! made it easy this week: vs. Please join by contributing your experience/question for a lively discussion!

  12. Jan13/2020 Question and Answer Forum (w/ and ). THIS WEEK: vs. -scRNAseq methods are optimal when cells can be harvested intact and viable.

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  13. 22. stu 2019.

    Single cell symposium Pros and cons of single nucleus RNA-seq from frozen tissues ____

  14. 8. stu 2019.

    Works like a charm. We’re really enjoying The possibilities are endless. 👩🏻‍🔬🧫🔬🧪👍🏻

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  16. Stereotyped transcriptomic transformation of somatosensory neurons in response to injury | ⁦

  17. DON’T FORGET to register (link below) to the second SCRUM! Room is filling up quickly!!! ZOOM will be available! also, sign up to get updates of coming events.!

  18. 20. lip 2019.

    Happy to share my first preprint on subthalamic nucleus!

  19. 24. svi 2019.

    Very good question....I also would like to store nuclei from FACS...

  20. 21. svi 2019.

    Up now! Eugene Drokhlyansky on human single-cell atlas using single-nucleus RNA-Seq

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