Rezultati pretraživanja
  1. 14. sij

    from my group developed hifiasm, a new assembler that preserves local phasing. Human asm in 1/2 day. Tested on non-humans. Comparable asm quality to others. Features in plan: consistent primary asm & global phasing. Feedback welcomed!

  2. 4. ruj 2019.

    PSA! HiFi = library prep & data type CCS = sequencing mode & read consensus analysis So, we'd say “I made a HiFi library, sequenced it in CCS mode, and ran the raw data through CCS analysis to get my for variant calling”. You’re welcome. ✌️

  3. 18. lis 2019.

    Awesome collaboration with Shilpa Garg and many others. Really the next level of fast and automated diploid human assemblies. Requiring and Hi-C reads. Awesome talk yesterday .

  4. 25. sij
    Odgovor korisnicima i sljedećem broju korisnika:

    Using the price list from (a CSP), a library prep and 30 hr Sequel II movie comes in right about $5000 for an external researcher. Arabidopsis can be run 5-7-plex, for a comparable or lower cost per sample

  5. 8. lis 2019.

    Evan Eichler talking about HiFi reads at AGTA2019. Game changer!

  6. 13. kol 2019.
  7. 15. sij
    Odgovor korisnicima

    And the make assemblies this large easier than ever. The bulk of the computation is the CCS job, which scales linearly with genome size and coverage. That replaces a massive preassembly job, which scales quadratically.

  8. 27. sij
    Odgovor korisnicima i sljedećem broju korisnika:

    Yep, definitely polish any CLR or ONT assembly, but it sure is nice to get it right the first time (and save a bunch of time, effort, and money) if you can design your experiment to use .

  9. 14. sij

    Happy to see there’s a new version of canu for by et al. That’s three new genome assemblers announced during . Hifi assembly is a game changer for genomics.

  10. 15. kol 2019.

    Oooh, want to put up against regular long read data in a human, an animal, and a plant?

  11. prije 10 sati

    Haplotype Diversity and Sequence Heterogeneity of Human Telomeres great preprint by from using

  12. prije 16 sati

    Exciting new study by resolving tandem repeats for evolutionary dynamics in Drosophila virilis. >40% of genome is a few related 7 bp satellites. No systematic errors in satellite sequences using :

  13. prije 17 sati
    Odgovor korisnicima i sljedećem broju korisnika:

    What assembler? Not sure there's a real solution outside of even more compute power with the data you have, unfortunately. You've come across one of the big advantages of , though. They let you swap the expensive preassembly calculation for a much more modest CCS job.

  14. 1. velj

    High Accuracy Long Reads open up new unexplored regions of the human genome: "We find that long telomeric stretches can be accurately captured with long-read [PacBio HiFi] sequencing". One step closer to Telomere-to-Telomere de novo assembly!

  15. 1. velj

    Registration is now open for - April 28-30. Join us, & the SMRT community for three days of engaging science & networking in the Netherlands. Featuring: Preview agenda & register:

  16. 22. sij

    Bonjour, ! Inspired by what you've heard so far? Learn more about on the new Sequel II System could accelerate your research: Or stop by and say hello to Deborah, Nicolas, Gerrit, Jonathan, et Eve!

  17. 16. sij

    Check out the blog summary of this recent preprint featuring fully phased human genome assemblies w/o trio sequencing: Strand-seq +

  18. 15. sij

    ICYMI - a great presentation from at on the latest version of his Canu genome assembler, now optimized for PacBio : HiCanu

  19. 15. sij

    What’s the future of deep variant? Cohort calling! Thanks

  20. 15. sij

    . shows that and deep variant out performs GATK and bwa-men for single nucleotide variants.

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