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@ChengChhy from my group developed hifiasm, a new#Hifireads assembler that preserves local phasing. Human asm in 1/2 day. Tested on non-humans. Comparable asm quality to others. Features in plan: consistent primary asm & global phasing. Feedback welcomed! http://github.com/chhylp123/hifiasm … -
@PacBio PSA! HiFi = library prep & data type CCS = sequencing mode & read consensus analysis So, we'd say “I made a HiFi library, sequenced it in CCS mode, and ran the raw data through CCS analysis to get my#HiFireads for variant calling”. You’re welcome.
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Awesome collaboration with Shilpa Garg
@lh3lh3@infoecho@MedhatHelmy7 and many others. Really the next level of fast and automated diploid human assemblies. Requiring#HiFireads@Pacbio and Hi-C reads. Awesome talk yesterday#ASHG19 .@BCM_HGSChttps://www.biorxiv.org/content/10.1101/810341v1 … -
Evan Eichler talking about
@PacBio HiFi reads at AGTA2019. Game changer!#millenniumscience#agta19#pacbio#hifi#hifireads pic.twitter.com/UmdG8u6aB4
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Using the price list from
@uwgenome (a@PacBio CSP), a#HiFiReads library prep and 30 hr Sequel II movie comes in right about $5000 for an external researcher. Arabidopsis can be run 5-7-plex, for a comparable or lower cost per sample https://pacbio.gs.washington.edu/?utm_source=Website&utm_medium=CSP-Referral&utm_campaign=title-link … -
And the
#HiFiReads make assemblies this large easier than ever. The bulk of the computation is the CCS job, which scales linearly with genome size and coverage. That replaces a massive preassembly job, which scales quadratically. -
Yep, definitely polish any CLR or ONT assembly, but it sure is nice to get it right the first time (and save a bunch of time, effort, and money) if you can design your experiment to use
#HiFiReads. -
Happy to see there’s a new version of canu for
@PacBio#HiFireads by@sergekoren et al. That’s three new genome assemblers announced during#PAGXXVIII. Hifi assembly is a game changer for genomics. -
Oooh, want to put
#HiFiReads up against regular long read data in a human, an animal, and a plant? https://twitter.com/drsarahdoom/status/1162109582774165504 … -
Still time to learn more about all things
@PacBio, especially#HiFiReads. Lanier A/B pic.twitter.com/hdFSnxuXvP
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Stop by the
@PacBio booth 1020 today to talk accurate and affordable long-read sequencing. Learn about#HiFiReads and our new Sequel II System.#ASHG19pic.twitter.com/9QXPdd8iR6
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JK: Yowza! This new 2.0 chemistry on the Sequel II is generating a LOT of 15kb
#HiFireads.#PBUGMpic.twitter.com/Dzjoxbna8y
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EH: Very recently, we’ve started to recommend
@PacBio#HiFiReads for de novo human genome assemblies that are both highly accurate and phased into haplotypes, with faster compute times! Talk to@zevkronenberg in the poster session (#1692) to find out more! pic.twitter.com/fKviSo8Zf9
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Shilpa Garg shows phased
#HiFiReads generate human assemblies with QVs > 55, and NG50 >25Mb. Methods on github. What an all star team!@PacBio@infoecho@EmilyHatas@jayghurye@lh3lh3@sedlazeck and more!#ASHG19 -
Hi Phil,
#HiFiReads are already released for Sequel 2. I like your circularity pun
. This website is a nice overview of the tech: https://www.pacb.com/smrt-science/smrt-sequencing/smrt-sequencing-modes/ … -
Check out the blog summary of this recent preprint featuring fully phased human genome assemblies w/o trio sequencing: Strand-seq +
#HiFiReads http://ow.ly/OH8i50xOKVd@tobiasmarschalhttps://twitter.com/Magdoll/status/1217578522304077826 …
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Well that’s that. Thanks to all who came to see us at
#ASHG19 to discover that@PacBio#SMRTSequencing can help advance your science with affordable, highly accurate long-read sequencing data you can trust.#HiFiReads pic.twitter.com/0Mc7hht5XN
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Well that all happened in the blink of an eye. Thanks to everyone who stopped by
@PacBio at#PAGXXVIII to talk#SMRTSequencing and#HiFiReads. See you next year! pic.twitter.com/vUzzjpW9AZ
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Haplotype Diversity and Sequence Heterogeneity of Human Telomeres https://www.biorxiv.org/content/10.1101/2020.01.31.929307v1 … great preprint by
@LankyCyril@jfoox from@mason_lab using@PacBio#HiFiReads
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