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#drosophila#connectomics w/@gsxej lab preprints!@SebaCachero@MRC_LMB developed + thoroughly characterised#BAcTrace to label neurons inputting those in your favourite genetic lines (opposite to Tango system). Results across many live flies ≈ those from one dead EM’d one https://twitter.com/fly_papers/status/1221311061690728448 …Prikaži ovu nit -
#BAcTrace, a#tweeprint: At least since Francis Crick’s famous review of 1979, neuroscientists have dreamed of ways to identify all the neurons connected to a cell of interest. In the last decade this has become a reality through the widespread use of rabies virus tracers. 1/11 https://twitter.com/biorxivpreprint/status/1221306607872757761 …pic.twitter.com/nVGKPvCexH
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We are grateful to long-term support from
@royalsociety (to@SebaCachero)@MRC_LMB and@ERC_Research. We will continue to refine the system in flies and we believe that#BAcTrace has promise in other organisms including fish and mice. 10/11 -
Flies will be deposited
@flystocks when the manuscript is accepted. Please contact us if you are interested in obtaining stocks ahead of time. We will ask that you send us feedback on how#BAcTrace works for you! 9/11 -
After almost 10y
#BAcTrace works well, but 1) you need a LexA driver for your receiver (upstream) neurons 2) high sensitivity means you will get a BAcTrace signal in neurons that express both the receiver and donor constructs – even if the overlap is at very low levels. 8/11 -
Although botox is a powerful toxin, we have engineered
#BAcTrace to be non-toxic to flies (and humans). This allowed us to make whole cell patch clamp recordings from connected neurons for at least 1-2 weeks after BAcTrace labelling. 7/11 pic.twitter.com/5YpvwC3ZkG
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We then tried a challenging test. Third-order ‘LHNs’ integrate stereotyped combinations of 5+ input glomeruli.
#BAcTrace revealed 12/16 connections expected from EM#connectomics data with a 10 synapse threshold. The top partner had >400 outputs onto the donor cells. 6/11pic.twitter.com/aeSGhvrzLf
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We first validated
#BAcTrace in two well characterised connections in the fly olfactory system. One was weak but stereotyped and highly selective (ORN-PN), the other is strong but characterised by random convergence of multiple cell types onto downstream neurons (PN-KC). 5/11 pic.twitter.com/q56e5gARcD
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In
#BAcTrace one genetic driver defines a *donor* population expressing an engineered C. botulinum neurotoxin (aka#botox!). This jumps backwards across the synapse to *receiver* neurons. The botox then releases a tethered transcription factor in the connected cells. 4/11pic.twitter.com/dn7XZ7SeSW
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We have developed a new fully genetically encoded tracing system,
#BAcTrace, and validated it in#Drosophila. This makes the fly the first animal with such systems for both retrograde and anterograde tracing (trans-Tango, TRACT). 3/11pic.twitter.com/p6i4Qav2lI
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https://tinyurl.com/y2623t3o
Estamos a tu orden para información adicional y presupuesto
E-mail: info@bioproducts.net

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#KPL#BacTrace®#patógenos#alimentos#perlas#magnéticas#inmunomagnética#DNase#moleculares#latinoamerica#Colombia#bpipic.twitter.com/35OyGLmKnl
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#Bactrace : Sylvester Stallone second rôle dans un nouveau Direct To Vidéo http://ow.ly/GE9M50veESO -
Qui regarde
#Bactrace avec@SlyStallone sur C+ ? -
Food Outbreak Sam's Club is Recalling Smoked Salmon for Listeria: http://wp.me/pkt1z-2NV via
@wordpressdotcom#BacTrace
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