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  1. Jan 26

    More w/ lab preprints! developed + thoroughly characterised to label neurons inputting those in your favourite genetic lines (opposite to Tango system). Results across many live flies ≈ those from one dead EM’d one

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  2. 28 Dec 2012

    Food Outbreak Sam's Club is Recalling Smoked Salmon for Listeria: via

  3. Jan 29

    , a : At least since Francis Crick’s famous review of 1979, neuroscientists have dreamed of ways to identify all the neurons connected to a cell of interest. In the last decade this has become a reality through the widespread use of rabies virus tracers. 1/11

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  4. Jan 29
    Replying to

    We have developed a new fully genetically encoded tracing system, , and validated it in . This makes the fly the first animal with such systems for both retrograde and anterograde tracing (trans-Tango, TRACT). 3/11

  5. Jan 29
    Replying to

    In one genetic driver defines a *donor* population expressing an engineered C. botulinum neurotoxin (aka !). This jumps backwards across the synapse to *receiver* neurons. The botox then releases a tethered transcription factor in the connected cells. 4/11

  6. Jan 29
    Replying to

    We first validated in two well characterised connections in the fly olfactory system. One was weak but stereotyped and highly selective (ORN-PN), the other is strong but characterised by random convergence of multiple cell types onto downstream neurons (PN-KC). 5/11

  7. Jan 29

    Although botox is a powerful toxin, we have engineered to be non-toxic to flies (and humans). This allowed us to make whole cell patch clamp recordings from connected neurons for at least 1-2 weeks after BAcTrace labelling. 7/11

  8. Jan 29

    After almost 10y works well, but 1) you need a LexA driver for your receiver (upstream) neurons 2) high sensitivity means you will get a BAcTrace signal in neurons that express both the receiver and donor constructs – even if the overlap is at very low levels. 8/11

  9. Jan 29
    Replying to and

    We are grateful to long-term support from (to ) and . We will continue to refine the system in flies and we believe that has promise in other organisms including fish and mice. 10/11

  10. Jan 29
    Replying to

    We then tried a challenging test. Third-order ‘LHNs’ integrate stereotyped combinations of 5+ input glomeruli. revealed 12/16 connections expected from EM data with a 10 synapse threshold. The top partner had >400 outputs onto the donor cells. 6/11

  11. Jan 29

    Flies will be deposited when the manuscript is accepted. Please contact us if you are interested in obtaining stocks ahead of time. We will ask that you send us feedback on how works for you! 9/11

  12. 29 Jul 2019

    : Sylvester Stallone second rôle dans un nouveau Direct To Vidéo

  13. 23 Sep 2019
  14. 13 Jun 2019

    Qui regarde avec sur C+ ?

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