1) https://blast.ncbi.nlm.nih.gov/Blast.cgi?PAGE=Proteins … this is the BLAST site. it is a US government run website that lets you search all known protein sequences for matches with other protein sequences.
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alright all you terrified dorks let me show you how to search for matching protein sequences because there is a CERTAIN GARBAGE PAPER OUT THERE
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2) you open this page up, put in the sequence you're interested in, then hit go
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so right now, we have an interest in a few CERTAIN SEQUENCESpic.twitter.com/XSGRPpCCye
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now if you type the first one of these, TNGTKR into blast and hit go....pic.twitter.com/WBLSzeyd5Y
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THIS is what you get. this is the top matches of every protein that matches the sequences you searched.pic.twitter.com/mDymJyrQrg
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BUT WAIT! you say THAT WAS A SHORT SEQUENCE MAYBE THAT ONE WAS A COINCIDENCE BUT WHAT ABOUT A LONGER ONE
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WOW I CANT BELIEVE HOW CLOSE THIS WUHAN VIRUS SEQUENCE IS TO.... A BAT CORONAVIRUSpic.twitter.com/n2C6HRwH7H
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GEEZ WOW IT'S A 100% MATCH TO .. tandem-95 repeat protein [Magnetospirillum magnetotacticum] AS WELL, MUST BE BIOENGINEERED THEN
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might i even point out that BLAST yells at you for searching short sequences
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congratulations, you are all now bioinformaticians ;) (please do not yell at me, actual bioinformaticians)
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one last thing: "wow, that's cool, but what do those other columns mean? E value sure sounds kinda scary!" good thing the BLAST website has you covered!pic.twitter.com/XhzkvD1SDn
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so for the first search of TNGTKR, the E values were over 15700. that means that we would expect to see 15700 matches to TNGTKR through RANDOM CHANCE ALONE
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anyway, i would appreciate it if any real bioinformaticians can chime in as well, as i haven't actually done any of this stuff in years
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