In case you missed it, I have set up a poll to assess popularity of #cas9 #delivery in #CRISPR experiments. Please have your say and retweet. Thanks.
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Please help spread the word,
#crispr#cas9 delivery poll.@Addgene@eLife@NatureBiotech@CRISPR_NewsPrikaži ovu nit -
Please
@idtdna and CRISPR guru@GaetanBurgio, could you retweet my#cas9 delivery poll. Cheers!Prikaži ovu nit -
Kym
@kmd6300 has kindly shared some results from a survey last year, 75 respondents. I should have done a proper survey focussing on many dimensions of the application.pic.twitter.com/QpFAIHNFD5
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2/2: Probably microinjection studies are into mRNA-based delivery but I think RNP would work equally good for them. This survey could not look into multidimensionality of the whole CRISPR method but I can include a detailed survey in the future blog.
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3/3 Just be wary of the fact that the following reagents are immune stimulatory (interferon up in transfected cells): IVT gRNAs Liposome delivery Unmodified mRNA (Cas9) dsDNA >> ssDNA Purified rCas9 protein & modified synthetic cr: tracrRNA DO NOT invoke immune response.
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Kraj razgovora
Novi razgovor -
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I voted “all plasmid” as my most used strategy, although my true favorite
is RNPs... good luck with this talk!! -
Thanks for voting..if I may ask, why are you guys shy on using RNP? Is it the cost, Cas9 purification, or ease with plasmids?
- Još 4 druga odgovora
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Really chuffed to see a huge response to my
#CRISPR#Cas9 poll. Hopefully final results will give insight into where we are heading into. Retweets and responses much appreciated.@crm_edinburgh@The_MRC@mrc_hgu@EdinburghUni . If your lab uses CRISPR/Cas9, please vote.Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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