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Another thing that isn't mentioned in the paper, are the reactions done with replicates? We would always run every qPCR reaction in double or triplicate. If the SD was too high the result was rejected.
The volume is almost certainly too high to permit controls like this. They automate as much of the process as possible (picrelated, what they have in Münster) and minimise staff interactions, & still most of the labs seem to be at capacity limits all the time.pic.twitter.com/DoQpuKn5ab
