I think the worst thing I've ever done was work in a neuroscience lab that experimented on animal subjects. The research output was practically worthless and the protocols were ghastly.
Sometimes in these circumstances it was possible to reattach the apparatus with emergency surgery, but usually we gave up and decapitated the bird over a sink. A quick death was probably better than going on in that condition, honestly.
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Other birds were used for cytological studies. This required brain slices; birds were obviously killed for this. However. The stain we used would bind to blood, so birds had to be exsanguinated.
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The easiest way to do this was to let the bird's heart pump blood out wholesale, so we'd anaesthetize them then slice open their chest, making sure to sever the aorta. Probably painless, but we would go home and drink in the dark after this procedure.
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