I think the worst thing I've ever done was work in a neuroscience lab that experimented on animal subjects. The research output was practically worthless and the protocols were ghastly.
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Sometimes in these circumstances it was possible to reattach the apparatus with emergency surgery, but usually we gave up and decapitated the bird over a sink. A quick death was probably better than going on in that condition, honestly.
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Other birds were used for cytological studies. This required brain slices; birds were obviously killed for this. However. The stain we used would bind to blood, so birds had to be exsanguinated.
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