The animals had to be alive for this, but they were deeply deeply and irreversibly anaesthetized, so while it was horrible to watch they didn't feel it. The in vivo monitoring was much worse I think.
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in the Andes falling into low spots in silver and mercury mines (full of CO2, because no air circulation, and CO2 is dense) and everyone standing at the side watching them suffocate. And this was the initial approach to killing Jews et al in Poland, but the executioners . . .
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all just quit on the spot after listening to people dying in the back of cars they were driving around. Anyway, it was like that. Watching this little bird desperately trying to breathe and thrashing around for 45s while it suffocated in a plastic box.
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Oh man, carbon monoxide for euthanasia was a no go for me. I saw it done once by someone who was too squeamish for cervical dislocation (worked with mice) and.... no. Brutal= quick.
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