oh I mean I'm okay but it did leave me pretty convinced that nearly all animal research is both evil and useless
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The animals had to be alive for this, but they were deeply deeply and irreversibly anaesthetized, so while it was horrible to watch they didn't feel it. The in vivo monitoring was much worse I think.
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The protocol here involved monitoring (i) certain discrete regions of the bird's brain, in real time, using implanted wires; and (ii) tracking their (sometimes silent) production of song by measuring air pressure in their lungs.
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