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Prikvačeni tweet
New
@biorxivpreprint on synapse-resolution reconstruction of motor control circuits in the Drosophila ventral nerve cord (VNC) led by@jasper_tms,@davidhildebrand, and Brett Graham. A great collaboration with the labs of@casa_tuthill &@janfunkey. 1/n https://www.biorxiv.org/content/10.1101/2020.01.10.902478v1 …pic.twitter.com/EDOPnvPKUR
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We are grateful for the support of
@AllenInstitute@IARPAnews@neurowitz@RJVneurotech@NINDSnews@NIMHgov@NIH & the efforts of those who made it possible including@aaron_t_kuan,@lathomas42, @TriNguy81821638,@julia_buhmann,@singlephotons, Brendan Shanny &@LuxelCorp. 11/endPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Our evidence suggests that bCS neurons are poised to drive fast, synchronous leg flexion in response to changes in load on the legs. We expect future functional experiments will further characterize the role of these neurons in behavior. 10/n
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Indeed, these sensory neurons specifically target motor neurons that control rapid flexion of the leg, but avoid synapsing onto motor neurons that produce slow movements. These motor neuron types were recently characterized by
@singlephotons et al (https://www.biorxiv.org/content/10.1101/730218v2 …). 9/npic.twitter.com/fFFI01vp3FPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
We wanted to know if bCS axons target their synapses to particular types of motor neurons. By registering the EM dataset to a standardized VNC atlas, we can compare any EM reconstructed neurons to neurons imaged with light microscopy. 8/npic.twitter.com/PRMYTnOalI
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One highlight was finding a class of sensory neurons with huge-diameter axons that synapse on specific leg motor neurons on both sides of the body. We believe these are bilateral campaniform sensillum (bCS) neurons that encode changes in the load on the legs. 7/npic.twitter.com/2qSfc974XM
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We found that most motor neurons had unique and recognizable branching patterns. This let us find left-right pairs of motor neurons that likely control the same muscle in opposite legs. Identifiability of motor neurons is a key advantage to studying motor control in the fly. 6/npic.twitter.com/CiqSUtQozn
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We reconstructed all the motor neurons controlling the legs, wings, halteres, and neck; as well as the axons of hundreds of sensory neurons projecting into the VNC from the body. We make these publicly available (see the link above) to serve as a foundation for future work. 5/npic.twitter.com/7CnT7ZhObT
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We built a GridTape-compatible electron microscope to image over 20 million synapse-resolution images in 2 months. You can see the aligned dataset yourself at https://catmaid.virtualflybrain.org/ Thanks to
@virtualflybrain for publicly hosting the dataset! 4/npic.twitter.com/v6HaIc4gL5Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
To reconstruct the connectivity of neuronal circuits underlying behavior, we developed GridTape to automate the collection of sections for transmission electron microscopy. We used GridTape to collect 4400 sections of a fly VNC in 28 continuous hours. 3/npic.twitter.com/qbOXqyBiwD
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The VNC has circuits that sense & control the fly's limbs, similar to the spinal cord. Although it accounts for ~1/3 of the central nervous system, our understanding of the anatomy and function of the VNC has lagged behind our understanding of the fly brain. vid E. Dickinson 2/npic.twitter.com/8NEAC2Sc7Q
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Wei-Chung Allen Lee proslijedio/la je Tweet
Very exciting, new horizons ahead! X-ray holography to explore neural tissue at the nanoscale with 3D isotropic resolution.
@id16a@esrfsynchrotronhttps://twitter.com/aaron_t_kuan/status/1134194234901245952 …
0:13Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Wei-Chung Allen Lee proslijedio/la je Tweet
Very excited to share my first scientific paper with the world! X-ray imaging of neural circuits. Included videos show off the beautiful data. https://www.biorxiv.org/content/10.1101/653188v1.supplementary-material … As a
#drosophila scientist, my favorite vids are 1 (fly brain) 4 (fly leg) and 5 (#neuralnetwork reconstructions)https://twitter.com/aaron_t_kuan/status/1134194234901245952 …
0:13Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
New preprint from my lab on X-ray imaging and
#connectomics. Wonderful collaboration between@APacureanu and Peter Cloetens at@esrfsynchrotron,@aaron_t_kuan,@jasper_tms,@lathomas42 and more. https://www.biorxiv.org/content/10.1101/653188v1 …https://twitter.com/aaron_t_kuan/status/1134194355244134400 …
0:07Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Tomorrow (11/6) morning at
#sfn I'll be presenting@aaron_t_kuan's work via dynamic poster (DP08). Stop by if you're interested in the structure of a microcircuit that supported sequence activity in parietal cortex.Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Wei-Chung Allen Lee proslijedio/la je Tweet
Paper is out! Whole-brain electron microscopy volume of adult fruit fly at synaptic resolution https://www.cell.com/cell/fulltext/S0092-8674(18)30787-6 … video abstract: https://youtu.be/OqWAIQRRq5E Download dataset + preconfigured
@catmaid tracing server at: http://temca2data.org Thanks again to everyone involved!pic.twitter.com/wlBku4w9fz
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Wei-Chung Allen Lee proslijedio/la je Tweet
#SciJob: A postdoc position is available in the Lee Lab at@harvardmed investigating neural circuits https://scien.se/2tuLO7THvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Wei-Chung Allen Lee proslijedio/la je Tweet
The Jeanne Lab is now open at Yale School of Medicine! We are studying circuit mechanisms of neural computation in Drosophila! Join us! http://www.jeannelab.org
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Wei-Chung Allen Lee proslijedio/la je Tweet
mosca lab is hiring! we have exciting new funding for a postdoc to study synapse development! come do great science. https://www.moscalab.org/join-us/ pic.twitter.com/HoHvIl1FRH
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Wei-Chung Allen Lee proslijedio/la je Tweet
Sometimes when an article looks a little too relevant to your work, it turns out to actually be about your work.http://www.nature.com/news/how-to-map-the-circuits-that-define-us-1.22437?WT.mc_id=TWT_NatureNews&sf104735522=1 …
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