Nice! How did you get to label a single cell?
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Thanks! A low dose of tamoxifen into a pregnant Hopx-creER::mTmG mouse labels a few, sparsely distributed GFP+ radial glia cells in the developing brain of the pups.
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Clonal labelling lead to some of the most remarkable images! Is this work in progress? Would love to see the data associated with this labelling.
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I agree! Clonal is awesome! These cortical clones were a bi-product in a published project, hidden away on supplementary figure 2D.https://www.cell.com/cell/fulltext/S0092-8674(19)30159-X …
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Beautiful images! What did you use to render the video?
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Thanks! It is a reconstruction of multiple z+stacks. Video was rendered using
@ImarisSoftware
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Gorgeous work!
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Thanks!
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This looks so cool! Is that membrane-targeted GFP?
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Thanks! Yeah it is from the mTmG mouse. It is great for visualizing cell morphology.
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