crisprflydesign

@crisprflydesign

Building and sharing CRISPR tools for Drosophila genome engineering. Also general CRISPR news, open science, fly stuff. Personal account of Fillip Port.

Vrijeme pridruživanja: kolovoz 2013.

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  1. Prikvačeni tweet
    13. svi 2019.

    Our new preprint is out! "A large-scale resource for tissue-specific CRISPR mutagenesis in Drosophila." Short summary thread below.

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  2. proslijedio/la je Tweet

    Wow, January 2020 was the warmest on record and greater than 3°C above the 1981-2010 climate average in Europe. The average global temperature was also the highest on record. 🔥 More info ():

    Global and Europe map of January 2020 surface air temperatures from ERA5
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  3. Our sgRNA line HD_CFD01270 targets Axin, the ortholog of human tumor-suppressor Axin1. Axin1 is frequently mutated in a variety of cancers, leading to overactive Wnt signaling. CRISPR mutagenesis in flies results in overgrowth and induction of Wnt target gene senseless 👇

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  4. proslijedio/la je Tweet
    3. velj

    I can only strongly congratulate and for their heroic effort! This adds to the power of reverse genetics in flies which has a strong basis with transgenic RNAi through efforts from long live the pioneer organism Drosophila

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  5. proslijedio/la je Tweet
    3. velj

    Congratulations!! Get HD CRISPR library stocks from - currently 1459 sgRNA lines plus 22 toolkit stocks (Cas9 and Cas9/Gal4). Generate loss-of-function mutations in in a germline restricted, ubiquitous or tissue specific fashion.

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  6. 3. velj

    Apparently there is such a thing as getting a paper accepted directly after first round of reviews. At least that's what just happened to our CRISPR library manuscript at . Thanks to the whole team! 🎉

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  7. proslijedio/la je Tweet
    1. velj

    Challenging myself to tweet more! I’ll be sharing a fun fact about , commonly known as fruit flies, every day in February. Let’s start !

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  8. proslijedio/la je Tweet
    29. sij

    The idea that we can predict what will be "relevant for patients" is counterintuitive to me (crystal balls dont work). Much of what medicine is now excited about (iPS/CRISPR etc) comes from investments in reproducible, well-controlled discovery research. Fund discovery science.

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  9. proslijedio/la je Tweet
    14. sij

    CRISPR-Cas13d induces efficient mRNA knock-down in animal embryos All right, first story from the Moreno-Mateos lab. A great collaboration with the . Do you wanna induce knock-down in zebrafish and other animal embryos? Take a look to it :)

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  10. proslijedio/la je Tweet
    9. sij

    Why did I just find out about this? Goniurellia tridens, the 3-in-1 fruit fly!

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  11. proslijedio/la je Tweet
    9. sij

    We are looking for two postdoctoral researchers to join our new project at and . Are you interested in creating novel genetic tools to study signalling, mitosis, etc. across biological scales? Deadline: 18 January. Please RT. Info:

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  12. proslijedio/la je Tweet
    6. sij

    Australia: you have just experienced the future.

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  13. 4. sij

    Climate tipping points - when climate change leads to release of massive amounts of CO2 stored in the environment.

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  14. proslijedio/la je Tweet
    29. pro 2019.

    Class 2 CRISPR Type IV enzymes () are a new class of RNA-targeting proteins. Little is known about guide RNA design for high targeting efficacy. Together with and +lab we quantified the effect of 29,000 guide RNAs: () 1/16

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  15. proslijedio/la je Tweet
    26. pro 2019.

    Heidelberg - not only a great place for but likewise a beautiful, inspriring city. Today the castle in a foggy, majestic & christmassy 💫 mood Merry Christmas from !✨🎄💫

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  16. proslijedio/la je Tweet
    20. pro 2019.

    48.9 degrees C on the farm (western Vic) yesterday. Cockies falling dead from the trees. People and wildlife cannot withstand the extremes we are already experiencing and this is just 1 degree of warming. We are on track for more than 4. 😔

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  17. 21. pro 2019.

    Sums up pretty well the political response to the climate crisis: The Australian PM flying to Hawaii for a holiday while devastating bushfires rage at home following the driest year on record.

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  18. 20. pro 2019.

    The Heidelberg CRISPR Fly Design Library is on Flybase. All sgRNA lines now show up in the 'transgenic constructs' tab of each gene report. Thanks for all your work on this and everything else you do.

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  19. 19. pro 2019.

    ... is in line with the known role of optix in regulating cell proliferation in the anterior compartment of the wing disc.

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  20. 19. pro 2019.

    Fun to confirm the activity of sgRNA lines by inducing very specific phenotypes. Here we tested our line 2306 targeting the transcription factor optix in the wing. Note the reduction in the area between L2 and margin (asterisk). This....

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  21. 19. pro 2019.

    Interested in studying Hedgehog signaling in sunny Nice? Then check out this postdoc position in the Therond lab.

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