researcher 
Come for 
stay for some cooking 
Was this made with some sort of precursor to Incucyte? It’s super gorgeous!
Vintage movie Sunday: Just found a 15 year-old time-lapse of growing hippocampal neurons I made during my PhD! Time in hours.pic.twitter.com/WsilLAwn8X
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It was with a regular scope (a confocal I think)... Things were not as sophisticated back in 2004!
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That’s so cool! How did you keep the cells alive for so long outside an incubator?
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Standard live-cell imaging equipment - plexiglass chamber around the stage of the scope that allows to keep the cells at 37°C, 5% CO2
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This might be obvious but what species and age of individual were these taken from?
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Thanks for the question - these are rat embryonic neurons (from the hippocampus region of the brain), filmed here after a few days in culture.
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Looks like some movies I made during my PhD work, except the neurons are a couple days older!
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You were with the OG! Banker timelapses are such classics
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Can I use this as an illustration on the course blog for "Computational Neuroscience" at William & Mary? I'll give photo credit, of course.…https://apsc450computationalneuroscience.wordpress.com
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Sure! Credit is Christophe Leterrier, INP, Marseille, France.
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Is there any data on how often the form autapses in Banker's set up vs in vivo?
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I don't know - I imagine it is simple from electrophysiology to know what is the relative importance of autapses on a given patched neuron, so it may be somewhere
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Thanks! I always wondered when doing cultures.
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Should you come across that piece of data about autapses, please share here. Much appreciated.
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Neurite outgrowth never gets old.
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That's ageless I guess. It is beautiful.
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Dope
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Astonished by those axons that come together and radically change behaviour.
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Thanks for sharing! Brings back memories.
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This is incredibly satisfying
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