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Testing everything... Some surprises between a "reimplemented" HiFi-SIM (left) and the original HiFi-SIM (right). Btw

@fluoroforce

split LUTs are great to assess background artifacts on SIM reconstructions

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CellComM Lab and 9 others

FocalPlane

@focalplane_jcs

6h

🌟Deadline incoming for our 2023 Image competition! Get your images to us by Monday 19 June for your chance to see your image on the cover of

@J_Cell_Sci

and win £100. Thanks to everyone that has already submitted images, we can’t wait to share them! focalplane.biologists.com/2023/05/15/foc

Previous winner of JCS image competition by Tejeshwar Rao called 'Trippy Tension Turtle Tailing Treats’, an artistically rendered montage of HUVECs plated on a multiplex Tension Gauge Tether.

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Pablo J. Sáez

@pj_saez

19h

Searching help for a colleague that uses

@FijiSc

in Mac, today I found an answer by

, but from 12 YEARS AGO 👏🏽 (and yep I still use PC ¯\_(ツ)_/¯ because some other software are only for Windows🤦🏽‍♂️)

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20h

Just spent 2 hours tweaking the input of HiFi-SIM for a single file and the perspective of batch-adapting a Matlab GUI is a bit too much

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SIMists! What are the current best options for open-source 2D-SIM/TIRF-SIM reconstructions? I need something reasonably fast and able to batch process hundreds of frames for live-cell and DL training 🚀

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and I are organizing a "beyond pretty pictures" #cellbio2023

@ASCBiology

subgroup! If you extract cell biological insight by analyzing microscopy images please consider submitting an abstract by August 1 🔥 ascb.org/cellbio2023/?_ Please RT

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Mr. Mayo-R of Minneapolis Sandwiches

@DaphSci

Jun 13

We need a robust tubulin stain. We have tried "Tubulin Tracker Green (488nm Taxol), but it leaks out so fast and hasn't worked well. Any advice for non-genetic and non-antibody MT imaging? #FluorescenceFriday

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Jun 13

After #MicroscopyMonday, it's #TrainingTuesday

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focalplane.biologists.com

FocalPlane

@focalplane_jcs

Jun 13

On #TechnicalTuesday we are highlighting

@maralampert

's post on tracking in napari. Mara describes different options, guides the user through the process & calls for feedback on the tools. focalplane.biologists.com/2023/06/01/tra ‘Bio-image Analysis with Napari' series:

Bio-image Analysis with Napari - FocalPlane

How to guides for common bioimage analysis methods. Author: Mara Lampert

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Dans la jungle des cellules artificielles, ces petites usines à répliquer le vivant

🤩 We're in

for an article on Synthetic cells with

and many others !

lemonde.fr/sciences/artic]

lemonde.fr

L’ambition de reconstituer les cellules, unités élémentaires du vivant, reste intacte, plus d’un siècle après les premières propositions en ce sens. Qu’elles visent à comprendre les rouages de la vie...

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Jun 12

#MicroscopyMonday: a lonely axon finally finding a friend (animated mosaic image of 15 SIM images)

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Carlos García

@carlosg91018370

Jun 12

🧠 Now Pytorch, Tensorflow 1 and 2 and Onnx models can be easily and efficiently run together in any Java application. #JDLL will make the use of Deep Learning more accessible to Java developers and imaging applications users.

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Sunday ride to paradise 🏖️🚴

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Jun 10

Block party at La Plaine! Featuring the karoakorale and the Galette sound system

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A.D.O.R. - One For The Trouble (Sha-Eaze Remix) [1994]

Jun 10

Featuring this absolute gem of a remix (and an intro line made famous by Fatboy Slim)

youtube.com

I don't make any money on my uploads.. If the actual owner of the music wants me to take it down from Youtube. Let me know. REACH OUT TO ME: CrateDigga.mp3 @...

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Your Saturday afternoon needs this golden era (I'm old enough to not call this "old-school") hip-hop mix from

@UGLYMACBEER

mixcloud.com

Ugly Mac Beer - Da Moo Mix

A l'occasion de la sortie de son dernier album "The valley of the kings", Ugly Mac Beer nous avait fait l'honneur d'un mix exclusif aux teintes très hip-hop old school. Tracklist: 1.The Valley Of The...

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NeuroCyto Lab 2023 pic outtakes 😅

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Julien Hiblot

@JulienHiblot

Jun 9

What a nice way to finish the week! 🎉Our latest paper is now available online in

@nchembio

. 📚🔬If you missed the pre-print and like biosensors, explore our innovative approach for designing and developing chemogenetic sensors. 💡Check it out here: nature.com/articles/s4158.

Quote Tweet

Julien Hiblot

@JulienHiblot

Nov 30, 2022

Do you want to change the color of your biosensor on demand? Still have a large dynamic range? Check our preprint on ChemoX! More about it in the thread! (1/7) Congratulation to the team, in particular the twitterless Lars Hellweg. biorxiv.org/content/10.110

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Lamb smash burgers and melon-avocado crudo with a good Bandol 👌

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Me coming home with the last ingredient needed to make that recipe I've been thinking about all week

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Andy Moore

@aaandmoore

Jun 9

In a recent overnight imaging session, I made a startling discovery... COS7 throw midnight dance parties. Who knew? These party animals are dressed in Vimentin-TagGFP (orange) and the ER marker SnapTag-Sec61b (blue) #FluorescenceFriday

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Time for

's ExM show at team meeting! Will he manage to expand the cannelé cakes?

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Jun 9

Siri what's the carbon footprint of a 731,229-character failure output

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Say what you want about Python efficiency but getting a 22,668-word error output from a 3-word instruction is quite a generative feat

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Koen J.A. Martens

@kjaMartens

Temporal analysis of relative distances (TARDIS) is a robust, parameter-free alternative to...

We created TARDIS (Temporal Analysis of Relative DIStances), a conceptually new way to analyse single particle tracking (spt) data. TARDIS analyses spt data robustly & parameter-free, and is especially good in complex scenarios (e.g. high-density): biorxiv.org/content/10.110 1/8

biorxiv.org

In single-particle tracking (spt), individual particles are localized and tracked over time to probe their diffusion and molecular interactions. Temporal crossing of trajectories, blinking particles,...

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Sabrina Simoncelli

@ssimoncelli

JOB ALERT📢: Do you love seeing single-molecules under the microscope 🔬? We have an exciting 2.5 year #postdoc position at the interface of single-molecule tracking and fluorescence lifetime imaging funded by the Chan Zuckerberg Initiative.

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Good news, I'm receiving spam again on my Uni email! Nature is healing

Quote Tweet

Day 2 with no university internet and email. For those who want to reach me by email, please use my gmail address: firstname.lastname@gmail.com (I haven't received any univ-amu emails since yesterday 8 am) egions-francetvinfo-fr.translate.goog/provence-alpes

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Day 2 with no university internet and email. For those who want to reach me by email, please use my gmail address: firstname.lastname@gmail.com (I haven't received any univ-amu emails since yesterday 8 am) egions-francetvinfo-fr.translate.goog/provence-alpes

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Positions in the new Ries lab in Vienna ⬇️

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Jonas Ries

@JonasRies

The Ries lab maxperutzlabs.ac.at/research/resea is excited to move to the @maxperutzlabs at the @viennabiocenter, one of Europe’s hotspots for Life Sciences. Join a very talented and fun team (maxperutzlabs.ac.at/news/open-posi):

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Only two ingredients needed to make the perfect grilled cheese sandwich: cheddar inside, mayonnaise outside. Can make it fancy with a third ingredient (kimchi is a favorite)

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Celestia

@junipermoonlite

Jun 5

YOU ARE MAKING A SANDWICH but you can only include 3 ingredients inside (the bread does not count). What are you using?

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💁🏻‍♂️: OK let's use this distraction-free day to test some processing! 🖥️ : Sorry no tethering, no 'conda install numba' for you

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I'd like to thank my team, collaborators, family, and 4G tethering

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Me: Today's the day let's resubmit! Lab's internet: Goes down

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Shahrnaz Kemal

@ShahrnazKemal

Why are these oligodendrocytes dying? 🧵 below on our new #bioRxiv paper! We investigate the connection between microtubules in oligodendrocytes and neurodegeneration. biorxiv.org/cgi/content/sh Cells on the right were incubated with TPPP fibrils. Annexin V labels apoptotic cells.

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Danglot

@LydiaDanglot

We are organizing with

@GDR_ImaBio

an international symposium in Paris dedicated to organoïds imaging and biosensors. Feel free to submit an abstract or a poster. Registration is free but mandatory : biosens-oids.sciencesconf.org

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FocalPlane

@focalplane_jcs

focalplane.biologists.com

Our latest #preprints post is now available on FocalPlane. This list highlights new preprints in #bioimage analysis. As always, let us know if there are any recent preprints that we have missed!

Microscopy preprints – bioimage analysis tools - FocalPlane

Microscopy preprints – bioimage analysis tools - Discussions

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Spatial redundancy transformer for self-supervised fluorescence image denoising

Looks like the preprint link is not the right one! Here it is:

biorxiv.org

Fluorescence imaging with high signal-to-noise ratios has become the foundation of accurate visualization and analysis of biological phenomena. However, the inevitable photon shot noise poses a...

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SRDTrans, a self-structured denoising approach – application agnostic, impressive results with STORM and 2-photon calcium imaging: biorxiv.org/content/10.110. Code available with a nicely detailed GitHub repo 👍 github.com/cabooster/SRDT

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