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Prikvačeni tweet
I am excited to share my latest work on
@biorxiv! Here we try to understand how the mSWI/SNF, or the BAF complex, promotes polycomb-mediated repression? https://www.biorxiv.org/content/10.1101/2020.01.29.925586v1.full.pdf … 1/nPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Christopher Weber proslijedio/la je Tweet
Distal repression BAF displacing polycomb Titrating dosage
#ChromatinHaiku https://www.biorxiv.org/content/10.1101/2020.01.29.925586v1 … Thread from@chmweber here: https://twitter.com/chmweber/status/1223109201880207360?s=20 …pic.twitter.com/lkDXAyvHM6
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I should give a shoutout to
@behnamnabet and colleagues here for sharing dTAG ligand before it was commercially available and for developing a degron approach that is unbelievably efficient and dosage-sensitive. dTAG is the gold-standard!Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Christopher Weber proslijedio/la je Tweet
Dark side of genetics are indirect effects and global effects through altered distribution. This is great study!
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Collectively, our data supports the model that BAF activity promotes distal repression by titrating polycomb dosage. We’re sending this out soon, so please let me know if you have any comments, suggestions, or criticism! Thanks for following along! 10/npic.twitter.com/d7wtaz07T9
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Finally, if our model is correct then polycomb overexpression should block Hox derepression when Brg1 is degraded. We overexpressed a minimal vPRC1 complex (most catalytic activity) and indeed find that it suppressed derepression! 9/npic.twitter.com/DvEDGR2ERW
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Is BAF controlling polycomb dosage? We made dTAG mESCs to degrade PRC1, PRC2, and both. We titrate polycomb protein dosage at the same time-point as our Brg1 degron. Voila, we find that intermediate degradation mirrors Brg1 derepression, but maximal is stronger! 8/npic.twitter.com/R2plM2941V
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We next looked at features associated with active chromatin (K4me3, K27ac, and ATAC) and find that they negatively correlated with polycomb changes. BAF doesn’t generate accessibility to load polycomb and is excluded from repressed domains. BAF isn't working bidirectionally. 7/npic.twitter.com/GchaW2K6hF
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Is this loss sufficient to decompact Hox clusters? We collaborated with the Boettiger lab to trace the DNA paths at HoxA and HoxD clusters with ORCA. Indeed, the polycomb loss is actually sufficient to physically decompact Hox! 6/npic.twitter.com/nAIpoGQPbW
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PRC1 and PRC2 are redistributed from highly bound domains to weakly bound sites genome-wide, consistent with driving the transcriptional response. 5/npic.twitter.com/RBIiAlbVDn
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Brg1 degradation rapidly derepresses many highly polycomb-bound genes in a time-dependent manner, such as Hox clusters and neurodevelopmental genes. So what happens to polycomb? 4/npic.twitter.com/SxKtnSJ989
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Traditional approaches (i.e. cKO/RNAi) take days to deplete proteins and cannot distinguish primary from secondary effects. To address this question directly, we created an mESC line where the ATPase subunit Brg1 can be rapidly degraded (T1/2 ~30m, near-complete ~2h). 3/npic.twitter.com/iuLl96rBFp
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BAF directly antagonizes polycomb-mediated repression from flies to humans (i.e. Trithorax Group). Yet, BAF somehow also promotes polycomb-mediated repression. How can BAF do both things? What’s the mechanism? 2/n
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Christopher Weber proslijedio/la je Tweet
Cool new study on how subunit switching of a chromatin remodeler controls neural cell fate! Great things come from
#UCSF and#Stanford collaborations!https://www.biorxiv.org/content/10.1101/2020.01.17.910794v1 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Ohhhh not that BAF, carry on.https://twitter.com/AliYoung05/status/1217176600606756864 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
I stumbled upon several fun interviews from scientists that I really look up to.
84th CSHL Symposium: RNA CONTROL & REGULATION https://www.youtube.com/playlist?list=PLCMsYfrNgAZNIbOjCcu8DVc_AFevxgqWY … via @YouTubeHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Anyone know why access to NCBI is restricted off-campus, but VPN works?
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Christopher Weber proslijedio/la je Tweet
Our preprint on splicing dynamics in human cells is now published with 5x more data! Congratulations to Heather and Karine! https://www.cell.com/molecular-cell/fulltext/S1097-2765(19)30865-2 … All
@nanopore data are here: https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE123191 … and all analysis scripts are deposited: https://github.com/churchmanlab/nano-COP …https://twitter.com/fiddle/status/1118189516106420224 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
I want to be like Toshi when I grow up!https://twitter.com/HutchBasicSci/status/1201981937235062784 …
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I’ve known Stephen for a long time. Superb mentor and lab. Seize the opportunity!https://twitter.com/NevesOnTheFly/status/1199741756981436416 …
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