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Dr. Angela Rasmussen
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Dr. Angela Rasmussen

@angie_rasmussen

Virologist at Columbia University. All about the host response to emerging pathogens and systems-level mind-blowing. And pugs. 1X Jeopardy! loser. she/her

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    Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
    • Prijavi Tweet

    Another (really good) preprint demonstrating that ACE2 can function as the receptor for #nCoV2019 #coronavirus. We need to be cautious about overinterpreting these types of virological data, with regard to understanding pathogenesis & possible treatments.https://www.biorxiv.org/content/10.1101/2020.01.31.929042v1 …

    11:38 - 1. velj 2020.
    • 25 proslijeđenih tweetova
    • 59 oznaka „sviđa mi se”
    • Pietro 🇺🇸🇮🇹 Steven Pergam, MD, MPH Ody_O Cole Trapnell ricercata Jason Kindrachuk Mark Przepiora Andras Toth 🇪🇺 feverfew
    5 replies 25 proslijeđenih tweetova 59 korisnika označava da im se sviđa
      1. Novi razgovor
      2. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        So, some background: A lot of CoVs (including SARS) use ACE2 as a receptor. The viral spike (S) glycoprotein binds ACE2 on target cells to get inside. Also S needs to be primed--basically clipped by an enzyme called a protease--to complete this process

        1 reply 2 proslijeđena tweeta 6 korisnika označava da im se sviđa
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      3. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        For SARS, one of these proteases is called TMPRSS2. This study shows that ACE2 and TMPRSS2 enable entry. In a host, ACE2 and TMPRSS2 expression determine what types of cells the virus can infect. This is called tropism.

        1 reply 1 proslijeđeni tweet 4 korisnika označavaju da im se sviđa
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      4. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        Tropism is important in terms of pathogenesis (the process of causing disease) because it determines what organs are infected. Viruses with broad cellular tropism are often (but not always) more pathogenic because they can spread to multiple tissues.

        1 reply 0 proslijeđenih tweetova 3 korisnika označavaju da im se sviđa
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      5. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        There was extrapulmonary spread reported in SARS patients, which might be relevant to #nCoV2019 if it uses the same receptors. So this study aimed to see if ACE2 and TMPRSS2 also mediated this process.

        1 reply 1 proslijeđeni tweet 4 korisnika označavaju da im se sviđa
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      6. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        The authors used a VSV pseudotype system. This is where the S protein from nCoV2019 is expressed on the surface of another virus called VSV. Inside the virion (viral particle), most of the VSV genome has been replaced by a reporter gene.

        1 reply 0 proslijeđenih tweetova 6 korisnika označava da im se sviđa
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      7. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        The S-pseudotyped VSV can bind the receptor (in this case ACE2 with TMPRSS2 priming) on a cell and enter, but once inside instead of the virus replicating, it expresses GFP and/or luciferase reporters. Reporter expression is then measured and used to quantify entry.

        1 reply 0 proslijeđenih tweetova 7 korisnika označava da im se sviđa
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      8. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        This system is widely used to study entry and the authors used it to look at nCoV2019 entry in a variety of cells expressing (or not) ACE2 and TMPRSS2. They also used a protease inhibitors, including a TMPRSS2 inhibitor, to investigate requirements for S priming

        1 reply 0 proslijeđenih tweetova 5 korisnika označava da im se sviđa
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      9. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        The experiments are well-controlled and indicate that ACE2 can act as a receptor and TMPRSS2 for priming. The data are convincing. HOWEVER. There are caveats.

        1 reply 1 proslijeđeni tweet 4 korisnika označavaju da im se sviđa
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      10. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        1. VSV pseudotyping systems and other in vitro studies must be interpreted in context. These are artificial viruses used to study entry in cell lines. This is very different than physiologically relevant circumstances in which nCoV2019 infects a person

        1 reply 0 proslijeđenih tweetova 5 korisnika označava da im se sviđa
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      11. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        2. Cell lines are almost always cancer cells or cells that have been immortalized by inserting genes that allow them to divide indefinitely. In this case, most experiments were done in HEK293T cells, which were transformed by 2 different viruses: an adenovirus & SV40 T antigen.

        1 reply 0 proslijeđenih tweetova 5 korisnika označava da im se sviđa
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      12. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        293Ts are great for transfection and protein expression. But they are NOTHING like cells in the human airway. They came from a human embryonic kidney, but it's also unclear what kind of cells they even are. Fibroblast? Epithelial? Neuronal? Who knows, they were made in the 1970s.

        1 reply 0 proslijeđenih tweetova 5 korisnika označava da im se sviđa
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      13. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        3. Receptor requirements are not the sole determinants of cellular tropism. These other factors include things like cellular antiviral responses (interferon signaling) or cell type-specific proteins or RNA that are required for virus genome replication or exit from the cell

        1 reply 0 proslijeđenih tweetova 3 korisnika označavaju da im se sviđa
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      14. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        4. ACE2 and TMPRSS2 can function for nCoV2019 cell entry but that doesn't mean they are the only proteins that can. The idea of using ACE inhibitors or TMPRSS2 inhibitors as antivirals is intriguing, but that doesn't mean they are guaranteed to be effective in patients

        1 proslijeđeni tweet 6 korisnika označava da im se sviđa
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      15. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        If the virus can use another protein as a receptor or another protease for S priming, then this doesn't matter. That doesn't mean it's a dumb idea, but we can't interpret studies done in vitro with tools for studying entry mechanism as promising preclinical data

        1 reply 0 proslijeđenih tweetova 2 korisnika označavaju da im se sviđa
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      16. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        To be clear the authors aren't suggesting that. They only state that a TMPRSS2 inhibitor approved for human use in Japan should be considered. They also interpret the data well in the context of the study and do not overstate their results.

        1 reply 1 proslijeđeni tweet 4 korisnika označavaju da im se sviđa
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      17. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        In fact, the most interesting part of the study (to me) is their last experiment, showing that serum from a SARS patient can block entry. This suggests that maybe people with preexisting immunity to SARS might be more resistant or SARS antibodies could be used to treat nCoV2019.

        3 proslijeđena tweeta 12 korisnika označava da im se sviđa
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      18. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        To their credit, the authors acknowledge the limitations of using serum from a single patient. They don't make wild assertions about the implications on tropism or what it might mean in terms of pathogenesis. I am impressed that the authors did a well-designed study so rapidly

        1 reply 0 proslijeđenih tweetova 5 korisnika označava da im se sviđa
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      19. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        However, I wanted to illustrate that critical mechanistic entry work like this is distinct from determining mechanisms of pathogenesis. It is one piece of a larger puzzle. To understand how nCoV2019 causes disease, we need data from animal models & clinical samples from patients.

        1 reply 1 proslijeđeni tweet 6 korisnika označava da im se sviđa
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      20. Dr. Angela Rasmussen‏ @angie_rasmussen 1. velj
        • Prijavi Tweet

        The bottom line is that these in vitro studies are critical to establish mechanism of entry, but more detailed studies need to be done with authentic nCoV2019 in vitro and in vivo before we can conclude anything about the significance for disease, treatment, or diagnostics

        1 reply 0 proslijeđenih tweetova 7 korisnika označava da im se sviđa
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