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Happy Chinese New Year! 新年快乐




Our lab celebrated with a dumpling-making party! 

(friends and colleagues in China: please stay healthy and safe!)pic.twitter.com/otwuQFhTjG
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Rad releases its inhibition of Ca+2 influx when cells see beta-agonist, explaining why the heart beats faster after adrenaline kicks in! (2/2) Congratulations to Steven Marx, Marian Kalocsay & colleagues!https://www.nature.com/articles/s41586-020-1947-z …
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Why does the heart beat faster during fight-or-flight response? This remarkable Nature paper performs APEX proximity labeling in beating mouse hearts (!!) to discover a new protein regulator (Rad) of voltage-gated Ca+2 channels. (1/2)https://www.nature.com/articles/s41586-020-1947-z …
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I am thoroughly enjoying Chris Walsh’s new textbook on the 13 human vitamins. Especially the chapters on my 2 most favorite vitamins, biotin and ascorbate. Highly recommended for anyone who loves metabolism and biosynthesis - a masterpiece of scholarship! https://pubs.rsc.org/en/content/ebook/978-1-78801-464-9 …
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Fascinated by mitochondria? Join us @ the Mito Biochemistry Keystone in Whistler! Speakers incl
@VamsiMootha@JLS_Lab J.Rabinowitz T. Schwarz@fiddle B.Tu M.Shuldiner@Pagliarini_Lab D.Chan@chembioBryan Scholarship deadline in 4 days! Abstracts by 2-19 http://tinyurl.com/s9q8pvfHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Cell-type specific surface proteome mapping in the developing fly brain
@Cell Terrific collab w/Liqun Luo. Co-1st dream team@JiefuBiol & Shuo Han Proximity labeling w/HRP & biotin-xx-phenol. 20 new protein regulators of synaptic wiring discovered.http://tinyurl.com/smerejzHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Congrats to grad student
@kelvinfcho on being one of 5 Stanford PhD students selected for a 2020 Blavatnik Fellowship! Kelvin is developing new technologies for detecting & mapping the molecular composition of cell-cell & organelle-organelle contacts (including split TurboID).Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Meeting website: http://bit.ly/KSmitobio20 Students & post-docs can apply for scholarships here (deadline is Jan 21): http://bit.ly/KSassistance
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I am co-chairing the Mitochondrial Biochemistry Keystone mtg in Whistler in May. All-star lineup incl
@VamsiMootha, Josh Rabinowitz,@chembioBryan, Cynthia Kenyon,@fiddle, Ben Tu, Tom Schwarz, Maya Shuldiner,@Pagliarini_Lab & many more! Students & post-docs encouraged 2 apply!pic.twitter.com/wrPhjYAwHT
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I tore my ACL playing tennis in Singapore over thksgiving & had ACL reconstruction surgery
@Stanford 2 wk later. Spent holiday recuperating w/support of family. So grateful for modern medicine & w/renewed appreciation for body & health. Such a privilege to walk, run, play sports!pic.twitter.com/YmEpERjGJO
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Directed evolution of “ultraTEV”, a faster variant of the biotechnological workhorse TEV protease, is out in Nature Methods. Tour de force by super-talented postdoc Mateo I Sanchez. Plasmids will be up on Addgene soon! https://www.nature.com/articles/s41592-019-0665-7 …https://twitter.com/naturemethods/status/1204124783132073984 …
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Alice Ting proslijedio/la je Tweet
Can two wrongs make a right? When it comes to mitochondrial dysfunction, the answer can be yes. See our new CRISPR compendium with
@broadinstitute@ScottVafai@SchreiberStuart@JohnDoench.https://www.cell.com/cell/fulltext/S0092-8674(19)31182-1#.Xc6TJRHkCNY.twitter …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
New study from Wade Harper using APEX proximity labeling to characterize the “mitochondria-autophagosome synapse” that forms upon mito depolarization, prior to mitophagy. Such comprehensive work, w/CRISPR-Cas9 screens also.
the schematics in Figs 4 & 6!http://tinyurl.com/yy72u7y5 Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Here’s a peek at the proteomic data. On the right are cell surface PN neuron proteins enriched in adult; left are enriched in pupae. Very cool to see known developmt proteins on left & synaptic transmission proteins on right. Grey dots most exciting - potential new discoveries!pic.twitter.com/FZExojv5lj
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Proximity labeling in pupae & adult fly brain reveals changes in neuronal surface proteome over development. New synaptic wiring candidates IDed and 20 validated by fly KD. Great collab w/Liqun Luo & Carr lab. Congrats co-1st authors
@JiefuBiol & Shuo Han!http://tinyurl.com/y2ep5qdwPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
TEV protease is widely used bc of its exquisite seq-specificity. We used directed evolution to improve its catalytic efficiency, employing light caging of TEV’s recognition seq to provide a kinetic selection. "ultraTEV" improves speed of FLARE & SPARK.http://tinyurl.com/yxbnuj2g
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Prime editing: a fundamentally new method for genome editing from
@davidrliu. Guided by single “pegRNA” w/ both guide and donor, a reverse transcriptase directly writes the edit into the genomic locus. No DSB & enables edits not prev possible. Stunning!http://tinyurl.com/y556a5x6Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
New work from Peng Zou et al. on light-activated proximity labeling of RNA (“CAP-seq”). Catalyst is miniSOG, which catalyzes RNA photooxidation that can be captured with propargyl amine and Click chemistry. New OMM biology. Congrats Zou & Wang labs!http://tinyurl.com/y3csjjc8
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Impressive study from Anne-Claude Gingras using proximity labeling to map the human spatial proteome. 192 BioID fusion constructs across 32 subcellular compartments produce localization data for >4K proteins. Data at http://humancellmap.org Preprint:https://www.biorxiv.org/content/10.1101/796391v1 …
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Thrilling & entertaining talk by
@francesarnold at the Codexis Protein Engineering Forum. New protein catalysts for making non-canonical aa's, C-Si bonds, b-lactams, C-H alkylation. Tremendous power in proteins; “Nature is just waiting for you to ask her” to release that power.Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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