Western Blot Facts

@WB_Facts

Painful facts from our favorite technique Tag us to share your frustration

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Vrijeme pridruživanja: siječanj 2016.

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  1. Prikvačeni tweet
    9. stu 2017.

    1. Prepare the samples 2. Cast the gels 3. Clean bench and recast gels 4. Realize there's no buffer 5. Prepare buffer 6. Run the gel 7. Realize there's no Gly 8. Borrow Gly 9. Transfer 10. Block 11. 1ary antibody 12. Wash 13. 2ary antibody 14. Wash 15. Develop 16. Fail 17. Goto 1

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  2. proslijedio/la je Tweet
    3. velj

    Western blot: Take 403

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  3. prije 11 sati
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  4. proslijedio/la je Tweet
    4. velj

    Cue screaming as I try to fit my Western blot around caucusing...

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  5. proslijedio/la je Tweet
    4. velj

    EL WESTERN BLOT MERECE MORIR EN UNA HOGUERAAAAAA 😡😡😡😡😭😭😭😭😭😭

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  6. proslijedio/la je Tweet

    My ggggg, I incorrectly connected my electrophoresis chamber and my western blot is destroyed. Throw the whole day away at this point because idek if I’ll have enough samples 😭😭🙄🙄

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  7. proslijedio/la je Tweet
    4. velj

    BLESS THE BIOLOGY LAB GODS my sdspage gels looked really good and its looking like my western blot is gonna come out nicely too 🔥🔥🔥

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  8. proslijedio/la je Tweet
    5. velj

    you've heard of the southern blot and the western blot but now get ready for the intermountain western blot: i vanish into a snowbank, never to be heard from again

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  9. proslijedio/la je Tweet
    prije 15 sati

    Passer 1 jour et demi sur un Western Blot pour se rendre compte au moment de la révélation que j’ai pas mis le bon anticorps secondaire : fait 🤡

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  10. proslijedio/la je Tweet
    2. velj

    I'm not sure how much I should read into the fact that my Western blot looks like *this*, but my Pothos propagations seem to have worked?

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  11. proslijedio/la je Tweet
    2. velj

    Never ever go to the lab to develop a western blot on a Sunday. It never ends well.

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  12. proslijedio/la je Tweet
    2. velj
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  13. proslijedio/la je Tweet
    2. velj

    Here’s another moment when western blot defeats you. Me: I run a damn good SDS-PAGE and western blot. Actin: HOLD ON THERE, CHIEF. I AM NOT WILLING TO COOPERATE TODAY!

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  14. proslijedio/la je Tweet
    Odgovor korisniku/ci

    Oh, I’ve learnt to never run a WB on a Friday...rule of thumb ;-)

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  15. proslijedio/la je Tweet

    How many times have you felt like this? (my awesome son in the photo)

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  16. proslijedio/la je Tweet
    31. sij

    Dear of twitter: Do you know what happened here? This detection of my went out terribly, while the and later stained membrane looked perfectly normal. I used two antibodys and SNAP i.d.

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  17. 1. velj
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  18. 1. velj
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  19. proslijedio/la je Tweet
    31. sij

    Wanna run a western blot? I definitely do not... But I do want to tell you about how they can be used to detect tiny amounts of specific proteins.      

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  20. proslijedio/la je Tweet
    31. sij

    You would think that given January has been approx 134 days long I could have figured out this Western blot

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  21. proslijedio/la je Tweet
    1. velj

    I never thought my biggest road block this past month would end up being the Western Blot. Fix one problem and then another pops up. Both negative stain preps went well, so there’s that.

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