Taco Jesse

@TJesse62

Disruptive Technologies & New Horizons - Views across the spectrum are reflecting my own.

The Netherlands
Vrijeme pridruživanja: prosinac 2014.

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  1. Prikvačeni tweet
    17. pro 2019.

    - Nanopore Sequencing has reached perfection This is a R9.4.1 after 24h ⁦

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  2. proslijedio/la je Tweet
    prije 6 sati

    Dawn of a new era for targeted sequencing ( ) : no enrichment prep, focus on enriching information during the run, bioinformatically. Only possible with

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    Towards real-time targeting/enrichment/other sampling on the sequencer, not in the sample prep: two new preprints from Kovaka/Payne show adaptive sampling using ‘Read Until’ API, find out more here: 1/3

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  4. proslijedio/la je Tweet
    prije 6 sati

    .'s write up on how adaptive sequencing works by controlling pore voltages in real time. Amazing that we can have so much control over individual molecules of DNA!

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  5. proslijedio/la je Tweet
    prije 6 sati

    Loaded ~600 ng on R10.3 and ~400 ng on R9.4.1.

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  6. proslijedio/la je Tweet
    prije 6 sati

    Sequencing first R10.3 batch. ~65% pores sequencing compared to ~85% on R9.4.1 using same library.

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  7. proslijedio/la je Tweet
    prije 6 sati

    Pick your panel poison, but things get much more interesting from here ..... dynamic assembly and gap filling on the fly ...... nicely done and crew 😊

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  8. proslijedio/la je Tweet
    prije 6 sati

    With it we show how you can reach 30x coverage of a 148 gene cancer panel on a single MinION run to yield SV calling 100% concordant to deep coverage ONT or HiFi reads. Crucially, we find twice the number of SVs using long reads compared to short reads in these cancer genes.

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  9. proslijedio/la je Tweet
    prije 6 sati

    Introducing "Targeted nanopore sequencing by real-time mapping of raw electrical signal with UNCALLED" with et al. Does selective enrichment and depletion of any targeted genomic region purely in software for sequencing.

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  10. proslijedio/la je Tweet
    prije 7 sati

    Read Until to enrich >700 genes to mean 30x coverage on single flowcell. Custom panels (up to 25,600 targets so far) as easy as providing a coordinate file. Read more at - thanks to , and team.

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  11. proslijedio/la je Tweet
    prije 6 sati

    Targeted nanopore sequencing by real-time mapping of raw electrical signal with UNCALLED

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    Building near-complete plant genomes - Current Opinion in Plant Biology

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  13. proslijedio/la je Tweet
    prije 6 sati
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  14. proslijedio/la je Tweet
    prije 15 sati

    EVENT - “One bite and the consumer is hooked”. Discover what cherry tomato Reddery RZ can mean for your business. Meet us during the Fruit Logistica - Hall 1.2 | D-04!

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  15. proslijedio/la je Tweet
    2. velj

    DELPHI: accurate deep ensemble model for protein interaction sites prediction

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  16. proslijedio/la je Tweet
    2. velj

    Fast and accurate assembly of Nanopore reads via progressive error correction and adaptive read selection

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  17. proslijedio/la je Tweet
    2. velj

    PacBio reads allowed to Improve Melon Genome assembly resulting in many young TEs detected and identification of genes that can be affected by TE insertion. Another example of the importance of long-read technologies for TE studies ⁦

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  18. proslijedio/la je Tweet
    24. sij

    A two-way molecular dialogue between embryo and endosperm is required for seed development

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  19. proslijedio/la je Tweet
    1. velj

    We had a great networking event with ~50 plant industry participants at our Utecht Plant Science & Industry Symposium UPSIS3 . Looking forward to future joint programmes.

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  20. proslijedio/la je Tweet
    31. sij

    Britain has a truly extraordinary record in biology, far above any other country’s and far more dramatic than in physics and chemistry. Genome editing is an alarming exception:

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  21. proslijedio/la je Tweet
    31. sij

    Joint statement on Brexit from EMBL, the Francis Crick Institute and the Wellcome Sanger Institute.

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