I’d like to thank Eric Betzig and Harald Hess for taking a chance on us for this important and super cool project. Working with them has been like going to see your favorite band in concert and unexpectedly the chance to play a song or two.
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@davephoffman, Gleb Shtengel and C. Shan Xu are masters at their trade and get the job done hands down. We’ve had the pleasure of working with David and Gleb the most: their rigor, stamina, high standards are a sight to behold. Photo = caught in the act freezing neuronspic.twitter.com/Qwew59wKBH
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@Daniel_Stabley (@EmoryChem &@salaitalab alum) opened the door to this project by mastering LLSM, helped shape key discussions of the#neuron project and pioneered@Blender_org#b3d for#sciviz. Visualization was key to unlocking the biology on this project and winning a coverpic.twitter.com/cl9PHDsl1o
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Kirby Campbell
@Kirb8989 (@UWMadison_BME &@UWMadisonLOCI alum) used LLSM to show condensing GNP nuclei & was tireless torture testing registration and analysis pipelines to make our CLEM data sing. His pipeline photo below is 1+ years of careful thought mixed w/pure grindpic.twitter.com/jATntrBUSS
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Abbas Shirinifard was the key to analysis in our part of the study. Who do you do to when you have GBs of super-res light data and FIB-SEM piling up in need of registration: the open source zen master
@fijisc@ilastik_team@3DSlicerApppic.twitter.com/QVfHKabG08
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There were many people that directly helped us set up the complex logistics of setting up a satellite granule neuron prep factory to support our part of the study.pic.twitter.com/PRPMXr8eQ3
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Hey, y'all. I wrote a layperson blog post for St. Jude to describe some parts of the CLEM study. Lessons learned: Be prepared for your opportunities You're only as strong as your team Keep at it even in failure URL: https://blogs.stjude.org/progress/new-microscopes-scientists-super-resolution/ … https://science.sciencemag.org/content/367/6475/eaaz5357 …pic.twitter.com/NhyCtr09ep
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You won't find this in the paper but we flipped the odometer on our Slack channel! Many thanks,
@SlackHQ we couldn't have done it without you!#collaboration#communication#ScienceTwitter#AcademicTwitter#AcademicChatterpic.twitter.com/CWrn5JcwOe
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By popular demand: here's a bit more cryo-CLEM public record:
@davephoffman kicks off freezing the first granule#neurons in 2017. There are so many CGNs in a cerebellum this was repeated MANY times#blizzard


#microscopy#science#cellbiologypic.twitter.com/tHlHdMHUZlPrikaži ovu nit -
Here's Gleb Freezing: he not only has amazing form but has the presence of mind to give a high-level Cryo lecture to us newbies. That's like going to the Bolshoi and getting a simultaneous dance history lecture by the performers.
#scicomm#ScienceTwitterpic.twitter.com/6vOBWElD55Prikaži ovu nit
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