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Prikvačeni tweet
Humbled to share our work w/ the Betzig and Hess labs
@HHMIJanelia made@Sciencemagazine cover Cerebellar granule#neurons gets a spot in the sun w/@Daniel_Stabley's render Not possible w/out support from@stjuderesearch@ALSAC@dnbresearch URL:https://science.sciencemag.org/content/367/6475/eaaz5357 …pic.twitter.com/HZ8KqGN3F3
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Isosurface
#rendering of CGN#neuron nucleus highlighting two heterochromatin types defined by HP1alpa and Histone3.3 overlap with classically defined heterochromatin that differ spatially and in abundance#sciart#Microscopy#chromatin#sciviz#sciencetwitter#AcademicTwitterpic.twitter.com/nFeii84PD4Prikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Kirby dissected whether these gross differences meant anything at the ultrastructural level and found the HP1 only labeled heterochromatin had much less lightly stained space in heterochromatin than the H3.3 only labeled materialpic.twitter.com/VZB5O0xWEy
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The cool thing abt the work is that SIM signal in the darkly stained classically defined
#heterochromatin parses this material in morphologically distinct areas HP1 only labeled areas tend to be at the nuclear periphery, while H3.3 only labeled tend to be in the interiorpic.twitter.com/M1YWCWD9eEPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Here’s
@blender_org render of the key morphological and surface area/vol differences between GNP & CGN nuclei that only can be detected by CLEM. Differentiation is comes with compaction of HP1 & H3.3 labeled heterochromatin and loss of euchromatin domain that lacks H3.3pic.twitter.com/CKNzqU5UafPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
There’s so many cool details to explore in the CLEM data sets: this darkly stained
#chromatin has core of HP1 Emerald but the periphery is labeled with H3.3 SNAPpic.twitter.com/dsxBi8wQ4U
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This is the GRAND tour of GNP & CGN nuclei showing off this CLEM data set in all it grandeur….still haven’t gotten anyone to dub the COSMOS theme song. Note GNP nuclei are not only larger than CGNs but differences are apparent for FIB-SEM plus HP1 Emerald & H3.3 SNAP SIM toopic.twitter.com/ann19G81KC
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Cryo-SR/EM CLEM was THE technique to study what changes in GNP & CGN in nuclear condensation
@davephoffman and Gleb collected cryo-SIM and 4 or 8 nm FIB-SEM data of cells w/HP1 Emerald & H3.3 SNAP Kirby and Abbas Shirinifard devised this pipeline to register & analyze CLEM datapic.twitter.com/8exdl7Kgf8
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We hypothesized that granule
#neuron progenitor (GNP) nuclei condensed during differentiation into granule neurons (CGN) Kirby@Kirb8989 used 12 hr long LLSM runs to prove it! Notice the GNP nucleus shrinks when blue Atoh1 marker txn factor disappears during CGN differentiationpic.twitter.com/jaS7DVXBqXPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Here’s a Figure 7 Cryo-SR/EM CLEM tweetorial for our
@sciencemagazine paper with@davephoffman@Daniel_Stabley@Kirb8989 We analyzed how#chromatin morphs in#neuron differentiation#microscopy#cellfie#cellbiology#AcademicTwitter#science URL: https://science.sciencemag.org/content/367/6475/eaaz5357 …pic.twitter.com/wLTzZxGxmuPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
David Solecki Lab proslijedio/la je Tweet
New preprint from the lab! So excited to get this cool story out there! Great work
@FabioUrbina4 . TRIM67 Regulates Exocytic Mode and Neuronal Morphogenesis via SNAP47https://www.biorxiv.org/content/10.1101/2020.02.01.930404v1 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
David Solecki Lab proslijedio/la je Tweet
Our third story for 2020. This is the work of the fantastic Alexandra and a fun collaboration with
@TheCesareLab and@ErosLazzerinihttps://www.biorxiv.org/content/10.1101/2020.01.31.929059v1 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
David Solecki Lab proslijedio/la je Tweet
And we're live! Big thanks to our collaborators
@KasperDHansen@loyalgoff @boukasleandros@cheminut & Gregg Semenza today up@JCI_Insight "Precocious neuronal differentiation and disrupted oxygen responses in Kabuki syndrome"#hypoxia https://insight.jci.org/articles/view/129375 …pic.twitter.com/4rZLD0APFZ
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David Solecki Lab proslijedio/la je Tweet
I am excited to share my latest work on
@biorxiv! Here we try to understand how the mSWI/SNF, or the BAF complex, promotes polycomb-mediated repression? https://www.biorxiv.org/content/10.1101/2020.01.29.925586v1.full.pdf … 1/nPrikaži ovu nitHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
I’m not
#complaining, but 70 degrees is freaking#weird on February 2.#SundayThoughtspic.twitter.com/W1j9UnnGM5
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David Solecki Lab proslijedio/la je Tweet
Wow!
@Daniel_Stabley keeps honing@blender_org for#science visualization: Loop to explore all facets of HP1 labeled heterochromatin revealed by 4nm FIB-SEM/CryoSIM from@davephoffman & co in the Betzig + Hess labs#sciart#sciviz#Sciencetwitter#biology#cellbiology#chromatinpic.twitter.com/o3z9XkYvafHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
David Solecki Lab proslijedio/la je Tweet
Very important paper!https://twitter.com/icaille1/status/1222871379084742657 …
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David Solecki Lab proslijedio/la je Tweet
This really is an awesome opportunity for imaging scientist to make an impact.https://twitter.com/SoleckiLab/status/1220021618023575552 …
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David Solecki Lab proslijedio/la je Tweet
Happy
#LunarNewYear2020! Here are some multispectral#organelle dynamics in a#rat#astrocyte (also happens to be my zodiac sign)pic.twitter.com/bmUizMzlT5Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Manuscript "shave" complete with just a "few" nicks. After deleting 15,000 characters hopefully, it's more concise and impactful
#AcademicTwitter#AcademicChatter#sciencetwitter#MyDeleteButtonFellOff#PaperWoespic.twitter.com/OHAvN0kemH
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David Solecki Lab proslijedio/la je Tweet
Centroid track of a segmented Entamoeba histolytica, fluorescenty labelled with Cell tracker. http://icy.bioimageanalysis.org/plugin/active-contours/ …pic.twitter.com/eVmwwg2kmv
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