David Solecki Lab

@SoleckiLab

Posting Solecki lab life and our science studying neurons with cool microscopes. Proud part of college

Memphis, TN
Vrijeme pridruživanja: rujan 2019.

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  1. Prikvačeni tweet
    16. sij

    Humbled to share our work w/ the Betzig and Hess labs made cover Cerebellar granule gets a spot in the sun w/'s render Not possible w/out support from URL:

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  2. prije 14 sati

    Isosurface of CGN nucleus highlighting two heterochromatin types defined by HP1alpa and Histone3.3 overlap with classically defined heterochromatin that differ spatially and in abundance

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  3. prije 16 sati

    Kirby dissected whether these gross differences meant anything at the ultrastructural level and found the HP1 only labeled heterochromatin had much less lightly stained space in heterochromatin than the H3.3 only labeled material

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  4. prije 16 sati

    The cool thing abt the work is that SIM signal in the darkly stained classically defined parses this material in morphologically distinct areas HP1 only labeled areas tend to be at the nuclear periphery, while H3.3 only labeled tend to be in the interior

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  5. prije 16 sati

    Here’s render of the key morphological and surface area/vol differences between GNP & CGN nuclei that only can be detected by CLEM. Differentiation is comes with compaction of HP1 & H3.3 labeled heterochromatin and loss of euchromatin domain that lacks H3.3

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  6. prije 16 sati

    There’s so many cool details to explore in the CLEM data sets: this darkly stained has core of HP1 Emerald but the periphery is labeled with H3.3 SNAP

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  7. prije 16 sati

    This is the GRAND tour of GNP & CGN nuclei showing off this CLEM data set in all it grandeur….still haven’t gotten anyone to dub the COSMOS theme song. Note GNP nuclei are not only larger than CGNs but differences are apparent for FIB-SEM plus HP1 Emerald & H3.3 SNAP SIM too

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  8. prije 16 sati

    Cryo-SR/EM CLEM was THE technique to study what changes in GNP & CGN in nuclear condensation and Gleb collected cryo-SIM and 4 or 8 nm FIB-SEM data of cells w/HP1 Emerald & H3.3 SNAP Kirby and Abbas Shirinifard devised this pipeline to register & analyze CLEM data

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  9. prije 16 sati

    We hypothesized that granule progenitor (GNP) nuclei condensed during differentiation into granule neurons (CGN) Kirby used 12 hr long LLSM runs to prove it! Notice the GNP nucleus shrinks when blue Atoh1 marker txn factor disappears during CGN differentiation

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  10. prije 16 sati
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  11. proslijedio/la je Tweet
    3. velj

    New preprint from the lab! So excited to get this cool story out there! Great work . TRIM67 Regulates Exocytic Mode and Neuronal Morphogenesis via SNAP47

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  12. proslijedio/la je Tweet
    2. velj

    Our third story for 2020. This is the work of the fantastic Alexandra and a fun collaboration with and

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  13. proslijedio/la je Tweet
    17. lis 2019.

    And we're live! Big thanks to our collaborators @boukasleandros & Gregg Semenza today up "Precocious neuronal differentiation and disrupted oxygen responses in Kabuki syndrome"

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  14. proslijedio/la je Tweet
    31. sij

    I am excited to share my latest work on ! Here we try to understand how the mSWI/SNF, or the BAF complex, promotes polycomb-mediated repression? 1/n

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  15. 2. velj

    I’m not , but 70 degrees is freaking on February 2.

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  16. proslijedio/la je Tweet
    19. pro 2019.

    Wow! keeps honing for visualization: Loop to explore all facets of HP1 labeled heterochromatin revealed by 4nm FIB-SEM/CryoSIM from & co in the Betzig + Hess labs

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  17. proslijedio/la je Tweet
    30. sij
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  18. proslijedio/la je Tweet
    29. sij

    This really is an awesome opportunity for imaging scientist to make an impact.

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  19. proslijedio/la je Tweet
    27. sij

    Happy ! Here are some multispectral dynamics in a (also happens to be my zodiac sign)

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  20. 26. sij

    Manuscript "shave" complete with just a "few" nicks. After deleting 15,000 characters hopefully, it's more concise and impactful😂

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  21. proslijedio/la je Tweet
    26. sij

    Centroid track of a segmented Entamoeba histolytica, fluorescenty labelled with Cell tracker.

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