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  1. 2. velj
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  2. proslijedio/la je Tweet
    31. sij

    Brutal!! 2A! the beast The cryo-EM Structure of Thermotoga maritima β-Galactosidase: Quaternary Structure Guides Protein Engineering

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  3. 31. sij

    I haven't tweeted politics much lately, but today is special. sums it up well below. Despite its nastiness in recent politics the UK remains an incredible place to do science, but tonight I will just drown my sorrows with & . ❤️🇪🇺😢🍺

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  4. 30. sij

    Main take-away point for microscopists, readers and editors: be wary of maps of that do not extend beyond 4A resolution. 😬 I know this for results, but probably same holds for other programs.

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  5. 30. sij

    Happy to see this out! In the Proceedings of the Spring Symposium in . Hopefully this will be useful for many who are doing (and other helical) reconstructions in .

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    Odgovor korisnicima i sljedećem broju korisnika:

    By the way, assuming ribosome's diameter is 300 A, Ewald sphere curvature starts to matter at around 2.1 A. It is worth trying Ewald sphere correction in relion_reconstruct or cisTEM.

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    A new section in RELION wiki explains when the pixel size of a reconstruction changes from the input, especially after CtfRefine's magnification refinement.

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    30. sij

    For a small fee and a collection of cited publications, anyone can file a challenged to a granted patent in the U.S. Veeerry interesting

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  9. 29. sij

    On my way to Sheffield to visit today! 😃

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  10. 29. sij
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    28. sij

    It is very inspiring to have my lab now in the same division that Crick first started in the LMB in 1962. The signed chalk board next to my lab is a reminder of his presence. He had such foresight!

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  12. 25. sij

    "They will also have to create the right spirit, something which may take time." I know of at least one place in the UK where that very spirit already exists: . Interesting fact: its unique research culture was established by a European immigrant: Max Perutz.

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  13. 24. sij
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    23. sij

    Smile! 📸 Group photo of attendees at the “wwPDB single-particle EM data-management workshop” . are glad world leaders in the community are here to debate EM data deposition & management. Thank you! 😀

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  15. 23. sij

    Loving the new term introduced by at the data management workshop today in Hinxton: the "Frankenstein map" 🧛‍♂️(which replaces the "composite map" for the combination of focussed refinement maps of different parts of a flexible complex)

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    22. sij

    These beam-image shifts definitely introduced aberrations. We could only get a structure of aldolase to 5.6Å initially (!). But, after repeated CTF refinement and particle polishing in , we could increase the resolution from 5.6Å to 2.8Å. Very impressive !

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    22. sij

    Please sign up for this year's Meeting UK in Edinburgh on 27th April members get a discount!

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  18. 22. sij
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  19. 20. sij
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  20. 20. sij

    A city’s best car plan is a bike plan! Hope more UK cities will follow. In Cambridge many people do cycle, but infrastructure and road culture are still lagging behind.

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