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We first validated
#BAcTrace in two well characterised connections in the fly olfactory system. One was weak but stereotyped and highly selective (ORN-PN), the other is strong but characterised by random convergence of multiple cell types onto downstream neurons (PN-KC). 5/11pic.twitter.com/q56e5gARcD
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In
#BAcTrace one genetic driver defines a *donor* population expressing an engineered C. botulinum neurotoxin (aka#botox!). This jumps backwards across the synapse to *receiver* neurons. The botox then releases a tethered transcription factor in the connected cells. 4/11pic.twitter.com/dn7XZ7SeSW
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We have developed a new fully genetically encoded tracing system,
#BAcTrace, and validated it in#Drosophila. This makes the fly the first animal with such systems for both retrograde and anterograde tracing (trans-Tango, TRACT). 3/11pic.twitter.com/p6i4Qav2lI
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