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1.- Our lab has identified a developmental mechanism underlying the exquisite precision of inhibitory connections in the brain. This is the story led by @emiliafavuzzi & @RubenDeogracias published @sciencemagazine (Thread) @dev_neuro @MRC_CNDD @KingsIoPNN @ERC @wellcometrustpic.twitter.com/MDg0KLjdr7
2.- In the cortex there are two main types of cortical neurons: principal neurons (excitatory) and #interneurons (inhibitory). The modulatory power of interneurons is greatly expanded by their ability to target different compartments of pyramidal cells.pic.twitter.com/1Sjdtuzc37
3.- How do these interneuron-specific connectivity motifs emerge during development? We identified cell-specific transcriptional dynamics in dendrite- (SST+), soma- (PV+) and axon- (chandelier) targeting interneurons when they are making their first synaptic contacts.pic.twitter.com/wTmf06lCXm
4.- Several type-specific molecular signatures emerge for the establishment of distinct inhibitory synapses and many of these genes are not type-specific at later stages.
5.- We found that whether interneurons #synapse on the dendrites, soma or axon initial segment of pyramidal cells is determined by synaptic molecules that are expressed in a subtype-specific manner.pic.twitter.com/ENsjUZWc7c
6.- We proved this by removing some of such synaptic molecules (Cbln4, Pcdh18, Lgi2, Fgf13) in a cell-specific manner and showing that their synaptic contacts were consequently altered.
7.- We also showed that overexpression of the SST+ cell-specific molecule Cbln4 in SST+ cells increases their dendritic synapses. However, expression of Cbln4 in PV+ or chandelier cells promotes the assembly of dendritic but not somatic or axo-axonic inhibitory synapses.pic.twitter.com/UZqD6pHNIg
8.- Thus, the selective expression of specific synaptic molecules in different interneurons promotes compartment-specific synapse formation.
9.- We do believe that better understanding the organizing principles of cortical inhibitory circuits will also help deciphering #neurodevelopmentaldisorders linked to inhibitory circuit dysfunction.
10.- Here’s the paper @sciencemagazine http://science.sciencemag.org/content/363/6425/413 … Thanks very much @ERC and @wellcometrust for funding and thanks all for sharing!
Congrats Beatriz, to you and the whole team - fabulous work!
Thanks very much!!!
Just starting reading this paper and our lab is loving it! We also enjoyed the ErbB4/CCK connectivity paper from a couple years back. So from one inhibition enthusiast to another, thanks for the cool science. 
Congrats Beatriz and Emilia! Fantastic paper!
Congratulations, awesome work!!
Congratulations! Very beautiful!
Fantastic work, any plans to test this in cells elsewhere in the brain?
How difficult would it be to mimic this in-vitro using iPSC-derived neurons? Is there a way to distinguish interneuron from an excitatory using staining?
Are these processed connected to neurodegenerative diseases?
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