Rabies has been engineered to act as a retrograde tracer, revealing all the inputs to a neuron. However limitations include toxicity and uncertainty about which synapses it crosses. Furthermore, these viruses are not applicable to all species. 2/11
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Odgovor korisniku/ci @gsxej
We have developed a new fully genetically encoded tracing system,
#BAcTrace, and validated it in#Drosophila. This makes the fly the first animal with such systems for both retrograde and anterograde tracing (trans-Tango, TRACT). 3/11pic.twitter.com/p6i4Qav2lI
1 reply 2 proslijeđena tweeta 13 korisnika označava da im se sviđa -
Odgovor korisnicima @SebaCachero @gsxej
In
#BAcTrace one genetic driver defines a *donor* population expressing an engineered C. botulinum neurotoxin (aka#botox!). This jumps backwards across the synapse to *receiver* neurons. The botox then releases a tethered transcription factor in the connected cells. 4/11pic.twitter.com/dn7XZ7SeSW
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Odgovor korisnicima @SebaCachero @gsxej
We first validated
#BAcTrace in two well characterised connections in the fly olfactory system. One was weak but stereotyped and highly selective (ORN-PN), the other is strong but characterised by random convergence of multiple cell types onto downstream neurons (PN-KC). 5/11pic.twitter.com/q56e5gARcD
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Odgovor korisnicima @SebaCachero @gsxej
We then tried a challenging test. Third-order ‘LHNs’ integrate stereotyped combinations of 5+ input glomeruli.
#BAcTrace revealed 12/16 connections expected from EM#connectomics data with a 10 synapse threshold. The top partner had >400 outputs onto the donor cells. 6/11pic.twitter.com/aeSGhvrzLf
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Although botox is a powerful toxin, we have engineered
#BAcTrace to be non-toxic to flies (and humans). This allowed us to make whole cell patch clamp recordings from connected neurons for at least 1-2 weeks after BAcTrace labelling. 7/11pic.twitter.com/5YpvwC3ZkG
1 reply 0 proslijeđenih tweetova 5 korisnika označava da im se sviđa -
After almost 10y
#BAcTrace works well, but 1) you need a LexA driver for your receiver (upstream) neurons 2) high sensitivity means you will get a BAcTrace signal in neurons that express both the receiver and donor constructs – even if the overlap is at very low levels. 8/111 reply 0 proslijeđenih tweetova 7 korisnika označava da im se sviđa -
Flies will be deposited
@flystocks when the manuscript is accepted. Please contact us if you are interested in obtaining stocks ahead of time. We will ask that you send us feedback on how#BAcTrace works for you! 9/111 reply 1 proslijeđeni tweet 10 korisnika označava da im se sviđa -
Odgovor korisnicima @gsxej @SebaCachero i sljedećem broju korisnika:
We are grateful to long-term support from
@royalsociety (to@SebaCachero)@MRC_LMB and@ERC_Research. We will continue to refine the system in flies and we believe that#BAcTrace has promise in other organisms including fish and mice. 10/111 reply 0 proslijeđenih tweetova 11 korisnika označava da im se sviđa -
Odgovor korisnicima @gsxej @sfrechter i sljedećem broju korisnika:
Many thanks to all authors including
@MarinaGkantia@as_bates@sfrechter Laura Blackie Amy McCarthy@sutcliffe_ben Alessio Strano Yoshi Aso@HHMIJanelia0 proslijeđenih tweetova 4 korisnika označavaju da im se sviđa
Love the multi-author thread...
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Odgovor korisnicima @RibeiroCarlitos @SebaCachero i sljedećem broju korisnika:
Alas
@threadreaderapp can’t unroll it0 proslijeđenih tweetova 3 korisnika označavaju da im se sviđa -
Odgovor korisnicima @as_bates @SebaCachero i sljedećem broju korisnika:
Yep only works on one author threads
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