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Researchers from
@OSUVetCollege used sophisticated technology, called#m6A#RNAsequencing, to identify the viral#gene that contains the most m6A#methylation.https://www.rna-seqblog.com/m6a-seq-exposes-a-viruss-hiding-place-and-reveals-new-potential-vaccine/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers from the
@NUAA_official developed#CircAST, a tool to assemble alternatively spliced#circRNA transcripts and estimate their expression on the basis of their internal structural characteristics.https://www.rna-seqblog.com/circast-full-length-assembly-and-quantification-of-alternatively-spliced-isoforms-in-circular-rnas/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
#Singlecell#RNAsequencing of individual parasite cells (#Tbruceibrucei) provides unique and high-resolution insights into the molecular processes that give rise to infective#metacyclicparasites transmitted at the host bite site. -@YaleSPHhttps://www.rna-seqblog.com/rna-seq-identifies-protein-that-could-help-neutralize-deadly-bite-of-the-tsetse-fly/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers at
@ugent present a comprehensive overview of the current#circRNA databases and their content, features, and usability.https://www.rna-seqblog.com/closing-the-circle-current-state-and-perspectives-of-circular-rna-databases/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers from the
@JohnsHopkinsSPH systematically evaluate the performance of 18 state-of-the-art#scRNAseq imputation methods using cell line and tissue data measured across experimental protocols.https://www.rna-seqblog.com/a-systematic-evaluation-of-single-cell-rna-sequencing-imputation-methods/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
While
#PCR amplification bias is often taken care by use of#UniqueMolecularIdentifiers (UMIs), normalization is required to remove effects of other technical variations like differences in#sequencingdepth, cell lysis and#reversetranscription efficiencyhttps://www.rna-seqblog.com/normalizing-single-cell-rna-sequencing-data-pitfalls-and-recommendations/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
A research team from the
@waynemedicine, the#CreateFertilityCentre and the@UMassSPHHS provides the first in-depth look at the#microbiome of human#sperm utilizing#RNAsequencing to identify contamination and#pathogenic bacterial colonization.https://www.rna-seqblog.com/study-provides-first-look-at-sperm-microbiome-using-rna-sequencing-sensitive-enough-to-detect-bacteria/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
To process large-scale
#singlecell#RNAsequencing (#scRNAseq) data effectively,@PennMedicine researchers have develped SHARP, an ensemble random projection-based#algorithm which is scalable to clustering 10 million cells. -@Pennhttps://www.rna-seqblog.com/sharp-hyper-fast-and-accurate-processing-of-single-cell-rna-seq-data-via-ensemble-random-projection/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers at
@UNC_Lineberger developed the publicly available workflow,#VirDetect, that detects#virus and laboratory vector sequences in#RNAseq samples with high specificity.https://www.rna-seqblog.com/virus-expression-detection-reveals-rna-sequencing-contamination-in-tcga/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Three methods for
#geneexpression profiling have now been combined to produce spatially defined#singlecell maps of developing human organs from limited sample material, overcoming a major hurdle in studying#humandevelopment.https://www.rna-seqblog.com/techniques-converge-to-map-the-developing-human-heart-at-single-cell-level/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
An international team of
#researchers from@PKU1898,@Tsinghua_Uni,@NNU_China,@xgenusco, and@UCSDSkaggsSOP, use bacterial#transposase Tn5 to construct#RNAseq libraries without second-strand synthesis.https://www.rna-seqblog.com/sherry-rna-sequencing-by-direct-tagmentation-of-rna-dna-hybrids/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers at
@USherbrooke use annotation-independent detection of#RNA reads generated using a#reversetranscriptase with low structure bias to identify#noncodingRNA.https://www.rna-seqblog.com/reducing-the-structure-bias-of-rna-seq-reveals-a-large-number-of-non-annotated-non-coding-rna/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
“Using that information along with
#RNAsequencing we were able to identify the#genes and#geneticpathways for the individual functions that make up the systems that keep the tree alive for so long." -@UNTnewshttps://www.rna-seqblog.com/researchers-discover-secret-of-longevity-in-thousand-year-old-trees/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
European researchers are at the forefront when it comes to combining
#singlecell technologies with analytical methods and computational tools to enable unprecedented characterization of human cells. -@humancellatlas -@CARTANAbiohttps://www.rna-seqblog.com/in-situ-rna-sequencing-iss-technology-will-be-applied-to-spatially-map-cell-types-for-international-human-cell-atlas-initiative/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Through
#RNAsequencing, scientists at@EdinburghUni &@dundeeuni found that a#probiotic called#Bacillussubtilis had a remarkable protective effect against the build-up of#alphasynuclein protein & also cleared some of the already formed protein clumps.https://www.rna-seqblog.com/researchers-use-rna-seq-to-understand-how-gut-bacteria-could-guard-against-parkinsons/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers from
@CONICETDialoga have developed the#NBSplice R package for#differentialsplicing analysis by means of#isoformexpression data. It estimates differences of#genetranscripts to infer changes in#gene#alternativesplicing patterns.https://www.rna-seqblog.com/nbsplice-differential-splicing-analysis-based-on-isoforms-expression/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers
@pfizer have developed 3’Pool-seq: a simple, cost-effective, and scalable#RNAseq method that focuses sequencing to the 3′-end of#mRNA. The researchers drew from aspects of#SMARTseq,#Dropseq, and#TruSeq to implement an easy workflow.https://www.rna-seqblog.com/3pool-seq-an-optimized-cost-efficient-and-scalable-method-of-whole-transcriptome-gene-expression-profiling/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers at
@KState compare experimental results using temporal controls and biological control types for time course#RNAseq drought stress experimentshttps://www.rna-seqblog.com/effects-of-control-type-choices-on-identifying-differentially-expressed-gene-for-time-series-rnaseq-experiments/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers at the
@kochinstitute developed a method for efficient detection of low-expression transcripts & a protocol specifically for T lymphocytes that links each cell’s#geneexpression profile with the sequence of its unique antigen receptor.https://www.rna-seqblog.com/technologies-to-watch-in-2020-single-cell-sequencing/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Researchers from
@riken_en Center for Biosystems Dynamics Research review the existing fast and memory-efficient#principalcomponentanalysis (PCA)#algorithms and implementations & evaluate their practical application to large-scale#scRNAseq datasets.https://www.rna-seqblog.com/benchmarking-principal-component-analysis-for-large-scale-single-cell-rna-sequencing/ …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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