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PrakashLab's profile
PrakashLab
PrakashLab
PrakashLab
@PrakashLab

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PrakashLab

@PrakashLab

Curiosity Driven Science

stanford.edu/~manup
Joined October 2011

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    PrakashLab‏ @PrakashLab Apr 16

    (1/n) Thrilled to share latest invention from our lab - a whole new way of vertical tracking microscope -capable of imaging single cells & marine larvae swimming as if in unconfined ocean? Read pre-print. Congratulations @deepak_me90 & #GravityMachine team https://www.biorxiv.org/content/10.1101/610246v1 …pic.twitter.com/E1ofxlvzis

    11:40 PM - 16 Apr 2019
    • 107 Retweets
    • 224 Likes
    • Alexey Guzey Ajay Aditya Chandramouli will_ludington Idearium Prahathees (Prah) Eswara Felix Woitzel Kunal Pandit Principal Scientific Adviser, Govt. of India Ellie Mackay
    3 replies 107 retweets 224 likes
      1. New conversation
      2. PrakashLab‏ @PrakashLab Apr 16

        (2/n) The project was lead by an absolutely incredible graduate student @deepak_me90 and also included "maker at heart" @hongquan_li, "software factory" Francois Benoit, Pierre Cambournac & "master-levitator" Adam Larson. This is really a dream team #GravityMachine. Congrats teampic.twitter.com/kOJ02PaAj0

        2 replies 1 retweet 25 likes
        Show this thread
      3. PrakashLab‏ @PrakashLab Apr 16

        (3/n) It all began with a paradox: can we observe microscopic processes (um scale) in ecological context (km)? We crack this problem - a scale-free vertical tracking microscopy - with 7 orders of magnitude range. Every time we turn it ON, our eyes are wide open! see proof below.pic.twitter.com/IrqxEj9je5

        3 replies 4 retweets 23 likes
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      4. PrakashLab‏ @PrakashLab Apr 16

        (4/n) This incredible journey to the depths of the ocean begins in swamps of Madagascar. More on that later! But first why should you care about life - so small its invisible to naked eye - in the ocean. Because 1/2 the oxygen you breadth comes from tiny plankton in the ocean.pic.twitter.com/pERKgphEOg

        1 reply 6 retweets 24 likes
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      5. PrakashLab‏ @PrakashLab Apr 16

        (5/n) Moreover, almost 1/2 half of the carbon emitted by our society is absorbed by the ocean - literally a process that keeps us alive - by marine detritus sinking several km to the bottom of the ocean. Ever wondered what happens during the long journey?pic.twitter.com/DWudXP80vh

        1 reply 4 retweets 28 likes
        Show this thread
      6. PrakashLab‏ @PrakashLab Apr 16

        (6/n) Several years ago, @deepak_me90 and I found ourselves in swamps of Madagascar, chasing parasites. We were neck deep in infested waters; and realized no tools existed to understand distribution of microscopic single cells at the scale of a pond, let alone the entire ocean.pic.twitter.com/3Ctog5PQcy

        2 replies 2 retweets 18 likes
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      7. PrakashLab‏ @PrakashLab Apr 16

        (7/n) Returning back we pondered over this question for a long time - and just like that; sitting in the office one day - @deepak_me90 and I cracked it. We realized a circular chamber filled with fluid has no beginning or end - hence was born a "hydrodynamic treadmill".pic.twitter.com/IuVF7uBlcJ

        1 reply 3 retweets 19 likes
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      8. PrakashLab‏ @PrakashLab Apr 16

        (8/n) Its one thing to come up with an idea; its another to believe in it to drop everything and execute. We all thought about this analytically for a while (see supplementary) - and played with plenty of hola loops to convince ourselves that it was time to build #GravityMachine.pic.twitter.com/853mHG5ua5

        2 replies 3 retweets 16 likes
        Show this thread
      9. PrakashLab‏ @PrakashLab Apr 16

        (9/n) Ever wondered how things fall? Say what happens to a raindrops falling down or pencil leads? Or a sugar cube falling and dissolving in your coffee right now - so as it disappear? Wonder no more, you can see it for yourself.pic.twitter.com/64pBxwSoZ9

        3 replies 4 retweets 27 likes
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      10. PrakashLab‏ @PrakashLab Apr 16

        (10/n) Next - we started by observing plankton and collected in the middle of the night (diel migration). Thanks to all the marine stations, fisherman that provided us with a roof, safety and free coffee; specially @HopkinsMarine and Isla Magueyes, Puerto Rico.pic.twitter.com/8DzTAYuiUV

        1 reply 3 retweets 18 likes
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      11. PrakashLab‏ @PrakashLab Apr 16

        (11/n) Here is a collection of a widest group of invertebrate larvae - watching them swim forever - as if in an unbounded ocean. Every time we put a new species, we are up for a surprise.pic.twitter.com/7BxRxty86h

        2 replies 6 retweets 23 likes
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      12. PrakashLab‏ @PrakashLab Apr 16

        (12/n) And what about the marine snow - microscopic form that is literally saving the planet. Here is a first glimpse ever of marine snow dynamics falling forever; you can watch the circulation, and also a radiolarian hitching a ride on a journey to the depth of the ocean.pic.twitter.com/8l9owpW0Zu

        1 reply 4 retweets 18 likes
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      13. PrakashLab‏ @PrakashLab Apr 16

        (13/n) Here is our favorite star; starfish P. miniate demonstrating stereotypical behavior - we call them blinks. Watching these videos, we are transported into an imaginary world sitting in a microscopic submarine, hitching a ride along these beautiful creatures.pic.twitter.com/65frtdRNZV

        1 reply 1 retweet 18 likes
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      14. PrakashLab‏ @PrakashLab Apr 16

        (14/n) Now what about the organisms that seemingly have no appendages to swim - say diatoms. The best way of describing this - @deepak_me90 and I always call this a "what the hell" slide. Incredible density fluctuations that governs height in tracked single diatoms.pic.twitter.com/D2Hnt2gSvE

        4 replies 4 retweets 29 likes
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      15. PrakashLab‏ @PrakashLab Apr 16

        (15/n) This paper was a tour-de-force; I want to extend the thanks for all the Prakazoans (what PrakashLab members are affectionally called) who actively participated tracking and collecting data - those long days tracking these datasets. Everyone still smiling after no sleep.pic.twitter.com/x0a7YRtHcI

        1 reply 2 retweets 28 likes
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      16. PrakashLab‏ @PrakashLab Apr 16

        (16/n) This is beginning of a long journey; we intend to bridge cell biology and marine ecology - for as many species as possible - from cells to invertebrate larvae. If you have a bizarre cell type you want to spy upon - feel free to reach out. Who knows what we will see?pic.twitter.com/UCCN1wERPY

        2 replies 5 retweets 41 likes
        Show this thread
      17. End of conversation
      1. New conversation
      2. Sina Booeshaghi‏ @sinabooeshaghi Apr 17
        Replying to @PrakashLab @deepak_me90

        Are the designs open source? There is some work being done at Caltech with clytia hemisphaerica and I think this could be pretty cool to build & use!

        1 reply 0 retweets 1 like
      3. PrakashLab‏ @PrakashLab Apr 17
        Replying to @sinabooeshaghi @deepak_me90

        I just got back from Caltech and we are working with Victoria Orphan lab. Please send a direct message and connect us.

        0 replies 0 retweets 1 like
      4. End of conversation
      1. New conversation
      2. MadScientist‏ @MadS100tist Apr 17
        Replying to @PrakashLab @deepak_me90

        WAIT. I just realized the one on the bottom is not an emoji

        2 replies 0 retweets 6 likes
      3. Amy Shaub Maddox‏ @AmyShaubMaddox Apr 17
        Replying to @MadS100tist @PrakashLab @deepak_me90

        Samesies!

        0 replies 0 retweets 2 likes
      4. End of conversation

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