Paolo Swuec

@PS_cryoEM

Cryo-EM Facility Manager @ UniMi | Will solve structure for food | Views expressed are my own

Milan, It
Vrijeme pridruživanja: rujan 2015.

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  1. Prikvačeni tweet
    5. lip 2019.

    Last month came to visit our lab to see how we are doing! Cool shots but my face couldn’t hide first nights with newborn 😅 ❄️🔬🇮🇹

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  2. prije 9 sati

    No students were harmed in the making of these grids. 😅 ❄️🔬

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  3. proslijedio/la je Tweet
    29. sij

    Works are underway on the North Pavilion which will host HT’s Cryo-Em microscopy and light microscopy imaging facility. 👷🏼‍♂️⚠️

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  4. proslijedio/la je Tweet
    28. sij

    The proof is in the -ing! So happy to finally have my first, first author paper go live as part of the special issue in BBA this month. Ever wondered if you could swap SMALPs for amphipols or detergents? Then this might be worth a look!😊

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  5. 16. sij

    So proud of Italian Ministry of Health celebrating Z-DNA on a 2€ coin...🤦‍♂️ !

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  6. 16. sij

    I need to specify this at least once a month (not only to new users!). 🤷‍♂️

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  7. proslijedio/la je Tweet

    When you use SIDESPLITTER, the followings seem to improve the resolution. - Start at relatively high res (10-15 A) - Use a very soft mask. A mask that cuts through a micelle is not good, even when it is soft. - Use solvent-flattened FSCs?: Yes - Mask particles with zeros: No

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  8. proslijedio/la je Tweet
    14. sij

    📢La comunità italiana di Biologia Strutturale è invitata a prendere parte al meeting di Instruct-ITALIA! Info: Registrazioni (partecipazione gratuita): 👉Please RT❗️

    , , i još njih 6
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  9. proslijedio/la je Tweet
    12. sij

    Apply with in Grenoble 🇫🇷 to join our growing EMBL community. You’ll also liaison with us here in Heidelberg 🇩🇪 as EMBL Grenoble collects data here to. Sharing is caring, so please RT.

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  10. 11. sij

    👌how did they measure a 140um holder shrink? Cooling it on stage and measuring grid slot diameter?

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  11. proslijedio/la je Tweet
    8. sij

    My new drawing for a paper! Anti- protein AcrIIA6, shown as handcuffs, sequesters and inhibits two Cas9 molecules (red), while a naturally resistant variant (green) is able to cleave its target DNA. Congrats , , , , et al!

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  12. proslijedio/la je Tweet
    3. sij

    A bash script that tabulates vital statistics of your 3d refinement in . Check quickly, how the refinement has progressed and if it has converged or not.

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  13. 31. pro 2019.

    Graphical abstract of my 2019. 🙏

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  14. proslijedio/la je Tweet
    27. pro 2019.

    Happy to finally share this work!! Proteasomes cluster at the ER, apparently by phase separation. We propose that these clustered proteasomes perform ERAD. They directly engage a specialized patch of the ER membrane and are surrounded by a cloud of Cdc48.

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  15. proslijedio/la je Tweet
    13. pro 2019.

    My first preprint! We wrote a new algorithm to fix overfitting in low resolution regions during SPA. It can even boost your resolution if your overfitting is serious. The code will be out soon, do give it a try!

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  16. proslijedio/la je Tweet
    15. pro 2019.

    Come to Melbourne to work with us on Correlative Light and Electron Microscopy. We have a position opening for a Senior Research Officer. Please share. microscopy

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  17. 13. pro 2019.

    It’s never too early to teach picking the right box size 😜 !

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  18. 5. pro 2019.

    Hey managers, what's best app/website/platform/service to share/store/edit protocols and GPL?!? (I know there's an EMBL holy grail WikiWim... what did you use?)

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  19. proslijedio/la je Tweet
    30. kol 2019.
    Odgovor korisniku/ci

    The best I can do is a screenshot of my notes. 😀

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  20. 4. pro 2019.

    Thinking aloud: EPU still applies beam-im shifts even when setting area in middle of the hole (tiny shifts but it does). So, am I refining an average beam-tilt in Relion?!? When do we start caring about what’s negligible for SPA?

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  21. proslijedio/la je Tweet
    4. pro 2019.

    Mark your calendars for the 2nd Cryo-EM User Symposium at EMBL Heidelberg on the 7th of February 2020. RT please!

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