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Prikvačeni tweet
Last month
@thermosciEMSpec came to visit our lab to see how we are doing! Cool shots but my face couldn’t hide first nights with newborn
https://youtu.be/2q0vs08V1HI 

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No students were harmed in the making of these grids.

pic.twitter.com/Mp9hlqoxfh
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Paolo Swuec proslijedio/la je Tweet
Works are underway on the North Pavilion which will host HT’s Cryo-Em microscopy and light microscopy imaging facility.

pic.twitter.com/wwo4CMCnGAHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Paolo Swuec proslijedio/la je Tweet
The proof is in the
#SMALP-ing! So happy to finally have my first, first author paper go live as part of the@smalpnet special issue in BBA this month. Ever wondered if you could swap SMALPs for amphipols or detergents? Then this might be worth a look!
https://doi.org/10.1016/j.bbamem.2020.183192 …pic.twitter.com/Z15yre4OL4
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So proud of Italian Ministry of Health celebrating Z-DNA on a 2€ coin...
@LHDNAHulk !pic.twitter.com/M60kzvqOU3
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I need to specify this at least once a month (not only to new users!).
https://twitter.com/SjorsScheres/status/1217805278927839232 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Paolo Swuec proslijedio/la je Tweet
When you use SIDESPLITTER, the followings seem to improve the resolution. - Start at relatively high res (10-15 A) - Use a very soft mask. A mask that cuts through a micelle is not good, even when it is soft. - Use solvent-flattened FSCs?: Yes - Mask particles with zeros: No
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Paolo Swuec proslijedio/la je Tweet
La comunità italiana di Biologia Strutturale è invitata a prendere parte al meeting di Instruct-ITALIA!
Info: http://bit.ly/2RapwTX
Registrazioni (partecipazione gratuita): http://bit.ly/2RapwTX
#NMR#cryoEM#structuralbiology#proteinproduction
Please RT
pic.twitter.com/8cJ6ZDVZN4
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Paolo Swuec proslijedio/la je Tweet
Apply with
@WojtekGalej@embl in Grenoble
to join our growing EMBL #cryoEM community. You’ll also liaison with us here in Heidelberg
as EMBL Grenoble collects data here to.
Sharing is caring, so please RT.https://twitter.com/WojtekGalej/status/1214561720997253120 …
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#Hagentorial
how did they measure a 140um holder shrink? Cooling it on stage and measuring grid slot diameter?https://twitter.com/WimJHH/status/1216066789668347904 …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
Paolo Swuec proslijedio/la je Tweet
My new drawing for a
@MolecularCell paper! Anti-#CRISPR protein AcrIIA6, shown as handcuffs, sequesters and inhibits two Cas9 molecules (red), while a naturally resistant variant (green) is able to cleave its target DNA. Congrats@AFMBLab,@doyonlab,@smoineau,@PS_cryoEM, et al!pic.twitter.com/r54LhzPpv6
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Paolo Swuec proslijedio/la je Tweet
A bash script that tabulates vital statistics of your 3d refinement in
#RELION. Check quickly, how the refinement has progressed and if it has converged or not. https://github.com/shahpnmlab/cryo-EM-bash-scripts/blob/master/ref3d_stats.sh …pic.twitter.com/9RKlxfvw4y
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Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Paolo Swuec proslijedio/la je Tweet
Happy to finally share this work!! Proteasomes cluster at the ER, apparently by phase separation. We propose that these clustered proteasomes perform ERAD. They directly engage a specialized patch of the ER membrane and are surrounded by a cloud of Cdc48. https://www.pnas.org/content/early/2019/12/26/1905641117 …pic.twitter.com/hciXClfGRs
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Paolo Swuec proslijedio/la je Tweet
My first preprint! We wrote a new algorithm to fix overfitting in low resolution regions during SPA. It can even boost your resolution if your overfitting is serious. The code will be out soon, do give it a try!https://twitter.com/cryoEM_Papers/status/1205558432394137600 …
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Paolo Swuec proslijedio/la je Tweet
Come to Melbourne to work with us on Correlative Light and Electron Microscopy. We have a position opening for a Senior Research Officer. Please share.
#CLEM#electron microscopyhttps://careers.pageuppeople.com/513/cw/en/job/599948/senior-research-officer-correlative-light-and-electron-microscopy#.XfbkLGzL71s.twitter …Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi -
It’s never too early to teach picking the right box size
!pic.twitter.com/VAS9CK7mvY
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Hvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Paolo Swuec proslijedio/la je TweetHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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Thinking aloud: EPU still applies beam-im shifts even when setting area in middle of the hole (tiny shifts but it does). So, am I refining an average beam-tilt in Relion?!? When do we start caring about what’s negligible for SPA?
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Paolo Swuec proslijedio/la je Tweet
Mark your calendars for the 2nd Cryo-EM User Symposium at EMBL Heidelberg on the 7th of February 2020. https://www.embl.de/services/cryo-em-platform/cryo-em-symposium/index.html … RT please!
#EMBLCryoEMHvala. Twitter će to iskoristiti za poboljšanje vaše vremenske crte. PoništiPoništi
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