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The tool checks if Android is able to send data through USB, which might not be possible if the cable is connected to the wrong port in the car, the cable is incompatible/damaged, or the phone's USB port is damaged.
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This is very true, Android Auto is quite picky about what cables work. An original Xiaomi cable had problems with my OnePlus 7 Pro and mi ex S9+ only allowed to use the Samsung cable, not even Ankers' . S21 Ultra works fine with the Xiaomi cable.
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