To do this, we use a combined light-sheet & virtual reality system, a two-photon system for single-cell ablation or excitation, and a computer cluster for fast processing of whole-brain data.pic.twitter.com/ksD1Ovlw75
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Our new paper in @naturemethods describes a system for function-guided perturbation of neural activity across the brain.
https://www.nature.com/articles/s41592-018-0221-x …
The goal of this work was to first measure for all neurons in the brain "what they do", then perturb a targeted subset of them.pic.twitter.com/UHjty2luvV
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After generating "brain activity maps" from light-sheet data, we use these maps to select neurons with specific functional properties (like stimulus tuning or correlation to motor output), and target them for cell-resolution ablation.pic.twitter.com/FMoe3efGvC
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We can then re-image whole-brain activity during behavior to determine the resulting changes in behavior and neuronal activity patterns (here, green = reduction in activity, magenta = increase, for six different ablations).pic.twitter.com/fufGspSRuh
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We extended the method to optogenetic excitation using an electrically-tunable lens-based optical scheme for targeting a set of single neurons during volumetric brain imaging.pic.twitter.com/jlgJkbTPTs
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Work by Nikita Vladimirov
@nvladimus, Burkhard Hoeckendorf, Chen Wang, and others, a very enjoyable collaboration with the lab of Philipp Keller, at@HHMIJanelia.Show this thread -
Software associated with the paper (https://www.nature.com/articles/s41592-018-0221-x …) is available at https://github.com/optofish-paper/ pic.twitter.com/U3dJjU54rO
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