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, and Oxford commas. Coincidences between my opinions and UW's are coincidental.
Thanks so much for writing this excellent review, @jakefwatson.
Prikaži ovu nit
A thirty-year-old breakthrough that went largely unnoticed. Short version: for most routine cloning applications we do not need to use restriction enzymes, ligases, Gibson, Gateway, etc. http://www.jbc.org/content/294/42/15271.full?sid=c047cc2b-3d2a-4182-8ed1-f82fd52b2110 …
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Like YRC but overhanging nonhomology cannot be removed
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Sort of. Needs less homology (15bp, often less) and is less influenced by internal homology (RecA-independent).
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This sounds crazy! Can you really do that with regular dh5a or 10b cells?
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Quikchange, really.
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As someone who does not do very much molecular work I've always been vaguely confused by what "cloning" actually means in contexts like this. Is it putting some sequence of interest into a plasmid for expression and/or propagation?
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Here’s a history of “molecular cloning” of this type; I don’t know who first used it - https://international.neb.com/tools-and-resources/feature-articles/foundations-of-molecular-cloning-past-present-and-future …
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where do I order the $1500 kit to do it though.
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