@pathogenomenick & HTS gurus-a Q:
Have U seen a drop in sensitivtiy when using RNA extracts prepared in presence of carrier RNA?
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So it definitely has a detrimental impact on some aspect?
@K_G_Andersen@pathogenomenick
Yes. I believe it's in our Genome Biol, Matranga et al we describe. cRNA DOES increase extraction, but not much
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I prefer using linear polyacrylamide (LPA) instead of cRNA so you don't have to deplete poly-A.
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In this instance, a kit uses cRNA. My question is whether that impacts negatively on NGS
@NathanGrubaugh@K_G_Andersen@pathogenomenick - Nog 2 antwoorden
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