A consensus on Zika virus sequencing strategies emerging from @K_G_Andersen http://virological.org/t/updated-phylogeography/362/13?u=n_j_loman …pic.twitter.com/89CN1pUmvH
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what do you find to be the major issues with SureSelect(ing) for zika in high ct samples?
We are still trying to optimize SureSelect. Main issues being pre-amplification required and lack of sensitivity.
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which SureSelect protocol are you using (200ng?)? Is preamplification in response to limited clinical material?
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Homebrew. But yeah, extremely limited starting material and we need ng - based on the Ct's it's closer to pg.
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