Has clinical SARSCoV2 testing been standardized yet? Field still seems noisy. What primer/probe combos have appear most sensitive and repeatable? This will guide assay selection for upcoming preclinical studies...any leads appreciated.
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Als antwoord op @RobertWCross1 @K_G_Andersen en
No. Still trying to get some data out on comparisons, but constantly slowed by our own surveillance work. CDC N1 and N2 work pretty well in tandem, though host cross reactivity happens. Berlin RdRp not very sensitive. Berlin E very good.
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Als antwoord op @NathanGrubaugh @RobertWCross1 en
We see N1 and N2 working pretty well too - comparable to the WHO assay. We're screening using CDC N1 and N2 followed by confirmation with the WHO assay after a positive hit.
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Als antwoord op @K_G_Andersen @RobertWCross1 en
What are you calling WHO, HKU? WHO now lists 4 assays on their website
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Als antwoord op @NathanGrubaugh @RobertWCross1 en
Not quite sure - Mark and Catie [not on twitter...] have the details. It's a three-amplicon assay.
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Als antwoord op @K_G_Andersen @RobertWCross1 en
The only one that I know has 3 is the “Berlin” assay from Drosten’s lab. UW started using that and switched to CDC due to issues with the RdRp sets (one primer set, 2 different probes). We are seeing the same issues with very high CTs due to mismatches
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Yup, PMID: 31992387. We're only using this second assay for validation of positive samples, so we wouldn't have run into those issues. The N1/N2 combo seems to be working well - hundreds of samples and no background. Not yet sure about 'real' sensitivity though. N3 is no good.
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