Has clinical SARSCoV2 testing been standardized yet? Field still seems noisy. What primer/probe combos have appear most sensitive and repeatable? This will guide assay selection for upcoming preclinical studies...any leads appreciated.
Not quite sure - Mark and Catie [not on twitter...] have the details. It's a three-amplicon assay.
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The only one that I know has 3 is the “Berlin” assay from Drosten’s lab. UW started using that and switched to CDC due to issues with the RdRp sets (one primer set, 2 different probes). We are seeing the same issues with very high CTs due to mismatches
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Yup, PMID: 31992387. We're only using this second assay for validation of positive samples, so we wouldn't have run into those issues. The N1/N2 combo seems to be working well - hundreds of samples and no background. Not yet sure about 'real' sensitivity though. N3 is no good.
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