See the debate on how to infer RNA virus origins from phylogenies: https://mbio.asm.org/content/10/2/e00289-19 … and the response. Interested parties should definitely check out the original alignments (especially Data Set S2) herehttps://mbio.asm.org/content/9/6/e02329-18 …
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Als antwoord op @edwardcholmes
The response by the authors.... so weird. Totally agree with you - that MSA is completely meaningless!
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Als antwoord op @K_G_Andersen
I thought these Letters were limited to 500 words?! I particularly liked the line about the median distance between the RTs and RdRps being 5 amino acid substitutions per site. Phew - that's alright then.
1 antwoord 0 retweets 2 vind-ik-leuks -
Als antwoord op @edwardcholmes
That's the funny thing - they started with laying out pretty well why it can't be done. Then went on to saying, "but we did it anyway". And then finished with, "but yeah, probably can't be done, but hey, whatever!"
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Als antwoord op @K_G_Andersen
More seriously, I am concerned that there is an increasing trend to infer phylogenies on highly divergent viruses that share almost no sequence similarity and where the alignment is dubious at best. Beware of crap alignments.
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Reminds me of arguments we had in the early 90s on whether potyvirus 3' UTRs had enough phylogenetic signal to group the viruses. Far fewer sequences, far fewer tools back then - but we had fun
@adrianjgibbs11 antwoord 0 retweets 5 vind-ik-leuks
“Back in the early 90s”. Fun times indeed. Didn’t have much to do with science back then on my part though 

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